Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging

Fumihito Miura, Yusuke Enomoto, Ryo Dairiki, Takashi Ito

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

DNA methylation plays a key role in epigenetic regulation of eukaryotic genomes. Hence the genome-wide distribution of 5-methylcytosine, or the methylome, has been attracting intense attention. In recent years, whole-genome bisulfite sequencing (WGBS) has enabled methylome analysis at single-base resolution. However, WGBS typically requires microgram quantities of DNA as well as global PCR amplification, thereby precluding its application to samples of limited amounts. This is presumably because bisulfite treatment of adaptor-tagged templates, which is inherent to current WGBS methods, leads to substantial DNA fragmentation. To circumvent the bisulfite-induced loss of intact sequencing templates, we conceived an alternative method termed Post-Bisulfite Adaptor Tagging (PBAT) wherein bisulfite treatment precedes adaptor tagging by two rounds of random primer extension. The PBAT method can generate a substantial number of unamplified reads from as little as subnanogram quantities of DNA. It requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes. Thus, the PBAT method will enable various novel applications that would not otherwise be possible, thereby contributing to the rapidly growing field of epigenomics.

Original languageEnglish
Pages (from-to)e136
JournalNucleic acids research
Volume40
Issue number17
DOIs
Publication statusPublished - Sep 1 2012
Externally publishedYes

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Genome
Epigenomics
DNA
hydrogen sulfite
5-Methylcytosine
DNA Fragmentation
DNA Methylation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging. / Miura, Fumihito; Enomoto, Yusuke; Dairiki, Ryo; Ito, Takashi.

In: Nucleic acids research, Vol. 40, No. 17, 01.09.2012, p. e136.

Research output: Contribution to journalArticle

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