Amplification of P450c21 expression in cultured mammalian cells

Michael H. Ricketts, Eric Chiao, Meng Chun Hu, Bon chu Chung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. With the exception of MMTV-LTR, all drove the expression of similar levels of functional 21-hydroxylase. In addition, the Rat-1 cell line was tested and shown to be suitable for the stable expression of functional P450c21. We have established cell lines derived from Rat-1 either normal or mutant P450c21 stably expressed together with amplifiable markers. The expression of P450c21 was further increased by selection in methotrexate.

Original languageEnglish
Pages (from-to)426-431
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number1
DOIs
Publication statusPublished - Jul 15 1992

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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