Amplification of receptor signalling by Ca2+ entry-mediated translocation and activation of PLCγ2 in B lymphocytes

Motohiro Nishida, Kenji Sugimoto, Yuji Hara, Emiko Mori, Takashi Morii, Tomohiro Kurosaki, Yasuo Mori

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

In non-excitable cells, receptor-activated Ca2+ signalling comprises initial transient responses followed by a Ca2+ entry-dependent sustained and/or oscillatory phase. Here, we describe the molecular mechanism underlying the second phase linked to signal amplification. An in vivo inositol 1,4,5-trisphosphate (IP3) sensor revealed that in B lymphocytes, receptor-activated and store-operated Ca2+ entry greatly enhanced IP3 production, which terminated in phospholipase Cγ2 (PLCγ2)-deficient cells. Association between receptor-activated TRPC3 Ca2+ channels and PLCγ2, which cooperate in potentiating Ca2+ responses, was demonstrated by co-immunoprecipitation. PLCγ2-deficient cells displayed diminished Ca2+ entry-induced Ca2+ responses. However, this defect was canceled by suppressing IP3-induced Ca2+ release, implying that IP3 and IP3 receptors mediate the second Ca2+ phase. Furthermore, confocal visualization of PLCγ2 mutants demonstrated that Ca2+ entry evoked a C2 domain-mediated PLCγ2 translocation towards the plasma membrane in a lipase-independent manner to activate PLCγ2. Strikingly, Ca2+ entry-activated PLCγ2 maintained Ca2+ oscillation and extracellular signal-regulated kinase activation downstream of protein kinase C. We suggest that coupling of Ca 2+ entry with PLCγ2 translocation and activation controls the amplification and co-ordination of receptor signalling.

Original languageEnglish
Pages (from-to)4677-4688
Number of pages12
JournalEMBO Journal
Volume22
Issue number18
DOIs
Publication statusPublished - Sep 15 2003
Externally publishedYes

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Lymphocytes
Phospholipases
Amplification
B-Lymphocytes
Chemical activation
Inositol 1,4,5-Trisphosphate Receptors
Inositol 1,4,5-Trisphosphate
Extracellular Signal-Regulated MAP Kinases
Cell membranes
Lipase
Immunoprecipitation
Transient analysis
Protein Kinase C
Visualization
Cell Membrane
Defects
Sensors

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Amplification of receptor signalling by Ca2+ entry-mediated translocation and activation of PLCγ2 in B lymphocytes. / Nishida, Motohiro; Sugimoto, Kenji; Hara, Yuji; Mori, Emiko; Morii, Takashi; Kurosaki, Tomohiro; Mori, Yasuo.

In: EMBO Journal, Vol. 22, No. 18, 15.09.2003, p. 4677-4688.

Research output: Contribution to journalArticle

Nishida, Motohiro ; Sugimoto, Kenji ; Hara, Yuji ; Mori, Emiko ; Morii, Takashi ; Kurosaki, Tomohiro ; Mori, Yasuo. / Amplification of receptor signalling by Ca2+ entry-mediated translocation and activation of PLCγ2 in B lymphocytes. In: EMBO Journal. 2003 ; Vol. 22, No. 18. pp. 4677-4688.
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