Amycolatopsis silviterrae sp. Nov., isolated from forest soil

Winai Jamjan, Chanwit Suriyachadkun, Somboon Tanasupawat, Kenji Sakai, Yukihiro Tashiro, Yuki Okugawa, Saowanit Tongpim

Research output: Contribution to journalArticle

Abstract

A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis. It contained MK-9(H4) as the predominant menaquinone, C16:0, iso-C15:0 and iso-C16:0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96%) and Amycolatopsis pretoriensis JCM 12673T (98.82%). The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9%) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8%). The DNA G+C content of strain C12CA1T was 69.8 mol%. Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp. nov. is proposed. The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).

Original languageEnglish
Article number002687
Pages (from-to)1455-1460
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number5
DOIs
Publication statusPublished - May 1 2018

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Amycolatopsis
forest soils
forest soil
Soil
phospholipid
DNA
Phospholipids
Diaminopimelic Acid
Vitamin K 2
Phosphatidylglycerols
Arabinose
Peptidoglycan
Glucosamine
Phosphatidylserines
Thailand
Phosphatidylinositols
Galactose
rRNA Genes
relatedness
Cell Wall

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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Amycolatopsis silviterrae sp. Nov., isolated from forest soil. / Jamjan, Winai; Suriyachadkun, Chanwit; Tanasupawat, Somboon; Sakai, Kenji; Tashiro, Yukihiro; Okugawa, Yuki; Tongpim, Saowanit.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 5, 002687, 01.05.2018, p. 1455-1460.

Research output: Contribution to journalArticle

Jamjan, Winai ; Suriyachadkun, Chanwit ; Tanasupawat, Somboon ; Sakai, Kenji ; Tashiro, Yukihiro ; Okugawa, Yuki ; Tongpim, Saowanit. / Amycolatopsis silviterrae sp. Nov., isolated from forest soil. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 5. pp. 1455-1460.
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abstract = "A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis. It contained MK-9(H4) as the predominant menaquinone, C16:0, iso-C15:0 and iso-C16:0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96{\%}) and Amycolatopsis pretoriensis JCM 12673T (98.82{\%}). The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9{\%}) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8{\%}). The DNA G+C content of strain C12CA1T was 69.8 mol{\%}. Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp. nov. is proposed. The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).",
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