Amycolatopsis silviterrae sp. Nov., isolated from forest soil

A novel actinobacterial strain, designated C12CA1^T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1^T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1^T was classified in the genus Amycolatopsis. It contained MK-9(H4) as the predominant menaquinone, C_16:0, iso-C_15:0 and iso-C_16:0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1^T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96%) and Amycolatopsis pretoriensis JCM 12673^T (98.82%). The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9%) and A. pretoriensis JCM 12673^T (8.8±0.3-9.2±1.8%). The DNA G+C content of strain C12CA1^T was 69.8 mol%. Based on the results of polyphasic characterization, strain C12CA1^T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp. nov. is proposed. The type strain is C12CA1^T (=TBRC 1456^T=NBRC 111116^T).