Amylibacter kogurei sp. Nov., a novel marine alphaproteobacterium isolated from the coastal sea surface microlayer of a marine inlet

Shu Kuan Wong, Susumu Yoshizawa, Yu Nakajima, Marie Johanna Cuadra, Yuichi Nogi, Keiji Nakamura, Hideto Takami, Yoshitoshi Ogura, Tetsuya Hayashi, Hiroshi Xavier Chiura, Koji Hamasaki

Research output: Contribution to journalArticle

Abstract

A novel Gram-negative bacterium, designated 4G11T, was isolated from the sea surface microlayer of a marine inlet. On the basis of 16S rRNA gene sequence analysis, the strain showed the closest similarity to Amylibacter ulvae KCTC 32465T (99.0 %). However, DNA-DNA hybridization values showed low DNA relatedness between strain 4G11Tand its close phylogenetic neighbours, Amylibacter marinus NBRC 110140T (8.0±0.4 %) and Amylibacter ulvae KCTC 32465T(52.9±0.9 %). Strain 4G11T had C18: 1, C16: 0and C18: 2as the major fatty acids. The only isoprenoid quinone detected for strain 4G11T was ubiquinone-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, one unidentified polar lipid, one unidentified phospholipid and one unidentified aminolipid. The DNA G+C content of strain 4G11T was 50.0 mol%. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the novel strain should be assigned to a novel species, for which the name Amylibacter kogurei sp. nov. is proposed. The type strain of Amylibacter kogurei is 4G11T(KY463497=KCTC 52506T=NBRC 112428T).

Original languageEnglish
Article number002911
Pages (from-to)2872-2877
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number9
DOIs
Publication statusPublished - Sep 1 2018

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Alphaproteobacteria
microlayer
alpha-Proteobacteria
Oceans and Seas
sea surface
DNA
rRNA Genes
lipid
Lipids
Phosphatidylglycerols
gene
isoprenoid
Terpenes
Base Composition
phospholipid
Gram-Negative Bacteria
Phosphatidylcholines
relatedness
Names
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Amylibacter kogurei sp. Nov., a novel marine alphaproteobacterium isolated from the coastal sea surface microlayer of a marine inlet. / Wong, Shu Kuan; Yoshizawa, Susumu; Nakajima, Yu; Cuadra, Marie Johanna; Nogi, Yuichi; Nakamura, Keiji; Takami, Hideto; Ogura, Yoshitoshi; Hayashi, Tetsuya; Chiura, Hiroshi Xavier; Hamasaki, Koji.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 9, 002911, 01.09.2018, p. 2872-2877.

Research output: Contribution to journalArticle

Wong, Shu Kuan ; Yoshizawa, Susumu ; Nakajima, Yu ; Cuadra, Marie Johanna ; Nogi, Yuichi ; Nakamura, Keiji ; Takami, Hideto ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Chiura, Hiroshi Xavier ; Hamasaki, Koji. / Amylibacter kogurei sp. Nov., a novel marine alphaproteobacterium isolated from the coastal sea surface microlayer of a marine inlet. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 9. pp. 2872-2877.
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abstract = "A novel Gram-negative bacterium, designated 4G11T, was isolated from the sea surface microlayer of a marine inlet. On the basis of 16S rRNA gene sequence analysis, the strain showed the closest similarity to Amylibacter ulvae KCTC 32465T (99.0 {\%}). However, DNA-DNA hybridization values showed low DNA relatedness between strain 4G11Tand its close phylogenetic neighbours, Amylibacter marinus NBRC 110140T (8.0±0.4 {\%}) and Amylibacter ulvae KCTC 32465T(52.9±0.9 {\%}). Strain 4G11T had C18: 1, C16: 0and C18: 2as the major fatty acids. The only isoprenoid quinone detected for strain 4G11T was ubiquinone-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, one unidentified polar lipid, one unidentified phospholipid and one unidentified aminolipid. The DNA G+C content of strain 4G11T was 50.0 mol{\%}. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the novel strain should be assigned to a novel species, for which the name Amylibacter kogurei sp. nov. is proposed. The type strain of Amylibacter kogurei is 4G11T(KY463497=KCTC 52506T=NBRC 112428T).",
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