An α-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe

Tomotake Morita, Naotaka Tanaka, Akira Hosomi, Yuko Giga-Hama, Kaoru Takegawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Glycosylphosphatidylinositol (GPI)-anchored proteins are essential for normal cellular morphogenesis and have an additional role in mediating cross-linking of glycoproteins to cell wall glucan in yeast cells. Although many GPI-anchored proteins have been characterized in Saccharomyces cerevisiae, none have been reported for well-characterized GPI-anchored proteins in Schizosaccharomyces pombe to date. Among the putative GPIanchored proteins in S. pombe, four α-amylase homologs (Aah1p-Aah4p) have putative signal sequences and C-terminal GPI anchor addition signals. Disruption of aah3 + resulted in a morphological defect and hypersensitivity to cell wall-degrading enzymes. Biochemical analysis showed that Aah3p is an N-glycosylated, GPI-anchored membrane protein localized in the membrane and cell wall fractions. Conjugation and sporulation were not affected by the aah3 + deletion, but the ascal wall of aah3Δ cells was easily lysed by hydrolases. Expression of aah3 alleles in which the conserved aspartic acid and glutamic acid residues required for hydrolase activity were replaced with alanine residues failed to rescue the morphological and ascal wall defects of aah3Δ cells. Taken together, these results indicate that Aah3p is a GPI-anchored protein and is required for cell and ascal wall integrity in S. pombe.

Original languageEnglish
Pages (from-to)1454-1463
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number6
DOIs
Publication statusPublished - Jul 4 2006
Externally publishedYes

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Glycosylphosphatidylinositols
Schizosaccharomyces
Amylases
Morphogenesis
Cell Wall
Membrane Proteins
Cells
Schizosaccharomyces pombe Proteins
Hydrolases
Proteins
Yeast
Defects
Glucans
Protein Sorting Signals
Aspartic Acid
Alanine
Saccharomyces cerevisiae
Glutamic Acid
Glycoproteins
Hypersensitivity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

An α-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe. / Morita, Tomotake; Tanaka, Naotaka; Hosomi, Akira; Giga-Hama, Yuko; Takegawa, Kaoru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 6, 04.07.2006, p. 1454-1463.

Research output: Contribution to journalArticle

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