An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress

Yoshio Kimura, Yukako Mishima, Hiromi Nakano, Kaoru Takegawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

An adenylyl cyclase gene (cyaA) present upstream of an osmosensor protein gene (mokA) was isolated from Myxococcus xanthus, cyaA encoded a polypeptide of 843 amino acids with a predicted molecular mass of 91,187 Da. The predicted cyaA gene product had structural similarity to the receptor-type adenylyl cyclases that are composed of an amino-terminal sensor domain and a carboxy-terminal catalytic domain of adenylyl cyclase. In reverse transcriptase PCR experiments, the transcript of the cyaA gene was detected mainly during development and spore germination. A cyaA mutant, generated by gene disruption, showed normal growth, development, and germination. However, a cyaA mutant placed under conditions of ionic (NaCl) or nonionic (sucrose) osmostress exhibited a marked reduction in spore formation and spore germination. When wild-type and cyaA mutant cells at developmental stages were stimulated with 0.2 M NaCl or sucrose, the mutant cells increased cyclic AMP accumulation at levels similar to those of the wild-type cells. In contrast, the mutant cells during spore germination had mainly lost the ability to respond to high-ionic osmolarity. In vegetative cells, the cyaA mutant responded normally to osmotic stress. These results suggested that M. xanthus CyaA functions mainly as an ionic osmosensor during spore germination and that CyaA is also required for osmotic tolerance in fruiting formation and sporulation.

Original languageEnglish
Pages (from-to)3578-3585
Number of pages8
JournalJournal of bacteriology
Volume184
Issue number13
DOIs
Publication statusPublished - Jun 24 2002

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Myxococcus xanthus
Osmotic Pressure
Germination
Spores
Adenylyl Cyclases
Signal Transduction
Genes
Sucrose
Reverse Transcriptase Polymerase Chain Reaction
Growth and Development
Cyclic AMP
Osmolar Concentration
Catalytic Domain
Amino Acids
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress. / Kimura, Yoshio; Mishima, Yukako; Nakano, Hiromi; Takegawa, Kaoru.

In: Journal of bacteriology, Vol. 184, No. 13, 24.06.2002, p. 3578-3585.

Research output: Contribution to journalArticle

Kimura, Yoshio ; Mishima, Yukako ; Nakano, Hiromi ; Takegawa, Kaoru. / An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress. In: Journal of bacteriology. 2002 ; Vol. 184, No. 13. pp. 3578-3585.
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