An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus

Yoshio Kimura, Mika Ohtani, Kaoru Takegawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have previously reported that a receptor-type adenylyl cyclase (CyaA) of Myxococcus xanthus undergoes an osmosensor mainly during spore germination (Y. Kimura et al., J. Bacteriol. 184:3578-3585, 2002). In the present study, we cloned another receptor-type adenylyl cyclase gene (cyaB) and characterized the function of the cyaB-encoded protein. Disruption of cyaB generates a mutant that showed growth retardation at high ionic (NaCl) or high nonionic (sucrose) osmolarity. When vegetative cells were stimulated with 0.15 M NaCl, the increases in intracellular cyclic AMP levels of cyaB mutant cells were lower than those of wild-type cells. Under nonionic osmostress, the cyaB mutant exhibited reduced spore germination; however, the germination rate of the cyaB mutant was significantly higher than that of the cyaA mutant.

Original languageEnglish
Pages (from-to)3593-3598
Number of pages6
JournalJournal of bacteriology
Volume187
Issue number10
DOIs
Publication statusPublished - May 1 2005
Externally publishedYes

Fingerprint

Myxococcus xanthus
Germination
Adenylyl Cyclases
Spores
Cyclic AMP
Osmolar Concentration
Sucrose
Growth
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus. / Kimura, Yoshio; Ohtani, Mika; Takegawa, Kaoru.

In: Journal of bacteriology, Vol. 187, No. 10, 01.05.2005, p. 3593-3598.

Research output: Contribution to journalArticle

Kimura, Yoshio ; Ohtani, Mika ; Takegawa, Kaoru. / An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus. In: Journal of bacteriology. 2005 ; Vol. 187, No. 10. pp. 3593-3598.
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