An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus.

S. Yasuda, M. Furuichi, E. Soeda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The DNA fragment encompassing the ras gene of Harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. Examination of nucleotide sequence, taken together with the result of analysis of the ras mRNAs (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. However, there were found several differences between the sequence published by Dhar et. al. (2) and ours, including 9 deletions, 7 substitutions and 2 insertions of nucleotides in the published sequence of 997 nucleotides in length. Among these, one of the substitutions occurring in the coding region resulted in amino acid replacement of glycine by alanine at position 122 of p21. The evidences are presented with some of actual gel autoradiographs.

Original languageEnglish
Pages (from-to)5583-5588
Number of pages6
JournalNucleic acids research
Volume12
Issue number14
DOIs
Publication statusPublished - Jul 25 1984

Fingerprint

Harvey murine sarcoma virus
ras Genes
Initiator Codon
Alanine
Glycine
Proteins
Nucleotides
Gels
Amino Acids
Messenger RNA
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus. / Yasuda, S.; Furuichi, M.; Soeda, E.

In: Nucleic acids research, Vol. 12, No. 14, 25.07.1984, p. 5583-5588.

Research output: Contribution to journalArticle

@article{2b6efca8d2584bd983f1e056d2daccd6,
title = "An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus.",
abstract = "The DNA fragment encompassing the ras gene of Harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. Examination of nucleotide sequence, taken together with the result of analysis of the ras mRNAs (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. However, there were found several differences between the sequence published by Dhar et. al. (2) and ours, including 9 deletions, 7 substitutions and 2 insertions of nucleotides in the published sequence of 997 nucleotides in length. Among these, one of the substitutions occurring in the coding region resulted in amino acid replacement of glycine by alanine at position 122 of p21. The evidences are presented with some of actual gel autoradiographs.",
author = "S. Yasuda and M. Furuichi and E. Soeda",
year = "1984",
month = "7",
day = "25",
doi = "10.1093/nar/12.14.5583",
language = "English",
volume = "12",
pages = "5583--5588",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "14",

}

TY - JOUR

T1 - An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus.

AU - Yasuda, S.

AU - Furuichi, M.

AU - Soeda, E.

PY - 1984/7/25

Y1 - 1984/7/25

N2 - The DNA fragment encompassing the ras gene of Harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. Examination of nucleotide sequence, taken together with the result of analysis of the ras mRNAs (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. However, there were found several differences between the sequence published by Dhar et. al. (2) and ours, including 9 deletions, 7 substitutions and 2 insertions of nucleotides in the published sequence of 997 nucleotides in length. Among these, one of the substitutions occurring in the coding region resulted in amino acid replacement of glycine by alanine at position 122 of p21. The evidences are presented with some of actual gel autoradiographs.

AB - The DNA fragment encompassing the ras gene of Harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. Examination of nucleotide sequence, taken together with the result of analysis of the ras mRNAs (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. However, there were found several differences between the sequence published by Dhar et. al. (2) and ours, including 9 deletions, 7 substitutions and 2 insertions of nucleotides in the published sequence of 997 nucleotides in length. Among these, one of the substitutions occurring in the coding region resulted in amino acid replacement of glycine by alanine at position 122 of p21. The evidences are presented with some of actual gel autoradiographs.

UR - http://www.scopus.com/inward/record.url?scp=0021770773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021770773&partnerID=8YFLogxK

U2 - 10.1093/nar/12.14.5583

DO - 10.1093/nar/12.14.5583

M3 - Article

C2 - 6087292

AN - SCOPUS:0021770773

VL - 12

SP - 5583

EP - 5588

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 14

ER -