TY - JOUR
T1 - An aluminum-resistance mechanism in eucalyptus camaldulensis
T2 - Complexation between aluminum and oenothein B in presence of organic acids in vitro
AU - Tahara, Ko
AU - Hiradate, Syuntaro
AU - Hashida, Koh
AU - Shinohara, Kenji
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science (JSPS) under a Grant-in-Aid for Scientific Research (KAKENHI; JP25850118) and by the Forestry and Forest Products Research Institute Encouragement Model in Support of Researchers with Family Responsibilities.
PY - 2017
Y1 - 2017
N2 - A hydrolyzable tannin, oenothein B, has recently been identified as a novel type of Al-binding and detoxifying ligand in a highly aluminum (Al)-resistant tree, Eucalyptus camaldulensis. Oenothein B is mainly localized in the root symplast, where the known ligands, citrate and oxalate, are also present. In this study, to investigate whether oenothein B can form complexes with Al in the presence of citrate and oxalate, we analyzed the chemical form of Al in multiple-ligand mixtures containing Al, oenothein B, citrate, and oxalate in vitro at concentrations mimicking those in the symplast of E. camaldulensis roots. In the mixture, 27% of total Al was detected in the insoluble fraction, and 28% in the high-molecular mass (> 10 kDa) soluble fraction. This means that more than half of Al in the mixture interacts with oenothein B, because among the three ligands only oenothein B can predominantly form insoluble or high molecular mass soluble complexes with Al. The27Al-nuclear magnetic resonance analyses of the mixture showed that low-molecular mass soluble Al was not mainly composed of simple Al–citrate or Al–oxalate complexes. These results support our hypothesis that oenothein B contributes to internal detoxification of Al in the symplast of E. camaldulensis roots.
AB - A hydrolyzable tannin, oenothein B, has recently been identified as a novel type of Al-binding and detoxifying ligand in a highly aluminum (Al)-resistant tree, Eucalyptus camaldulensis. Oenothein B is mainly localized in the root symplast, where the known ligands, citrate and oxalate, are also present. In this study, to investigate whether oenothein B can form complexes with Al in the presence of citrate and oxalate, we analyzed the chemical form of Al in multiple-ligand mixtures containing Al, oenothein B, citrate, and oxalate in vitro at concentrations mimicking those in the symplast of E. camaldulensis roots. In the mixture, 27% of total Al was detected in the insoluble fraction, and 28% in the high-molecular mass (> 10 kDa) soluble fraction. This means that more than half of Al in the mixture interacts with oenothein B, because among the three ligands only oenothein B can predominantly form insoluble or high molecular mass soluble complexes with Al. The27Al-nuclear magnetic resonance analyses of the mixture showed that low-molecular mass soluble Al was not mainly composed of simple Al–citrate or Al–oxalate complexes. These results support our hypothesis that oenothein B contributes to internal detoxification of Al in the symplast of E. camaldulensis roots.
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U2 - 10.1080/13416979.2017.1326656
DO - 10.1080/13416979.2017.1326656
M3 - Article
AN - SCOPUS:85047102463
VL - 22
SP - 261
EP - 264
JO - Journal of Forest Research
JF - Journal of Forest Research
SN - 1341-6979
IS - 4
ER -