An analysis of the mechanism of the low-wave phenomenon of chlorophyll fluorescence

Michito Tsuyama, Masaru Shibata, Tetsu Kawazu, Yoshichika Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The low-wave phenomenon, i.e., the transient drop of yield of modulated chlorophyll fluorescence shortly after application of a pulse of saturating light, was investigated in intact leaves of tobacco and Camellia by measuring fluorescence, CO2 assimilation and absorption at 830 nm simultaneously. Limitations on linear electron flow, due to low electron acceptor levels that were induced by low CO2, induced the low waves of chlorophyll fluorescence. Low-wave amplitudes obtained under different CO2 concentrations and photon-flux densities yielded single-peak curves when plotted as functions of fluorescence parameters such as ΦPS II (quantum yield of Photosystem II) and qN (coefficient of non-photochemical quenching), suggesting that low-wave formation depends on the redox state of the electron transport chain. Low waves paralleled redox changes of P700, the reaction center of Photosystem I (PS I), and an additional electron flow through PS I was detected during the application of saturating pulses that induced low-waves. It is suggested that low waves of chlorophyll fluorescence are induced by increased non-photochemical quenching, as a result of the formation of a trans-thylakoid proton gradient due to cyclic electron flow around PS I.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalPhotosynthesis Research
Volume81
Issue number1
DOIs
Publication statusPublished - Jul 1 2004

Fingerprint

Chlorophyll
Fluorescence
fluorescence
chlorophyll
electrons
Electrons
Photosystem I Protein Complex
photosystem I
Oxidation-Reduction
Camellia
Thylakoids
Quenching
Photosystem II Protein Complex
electron transport chain
Electron Transport
Photons
thylakoids
photosystem II
protons
Tobacco

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

An analysis of the mechanism of the low-wave phenomenon of chlorophyll fluorescence. / Tsuyama, Michito; Shibata, Masaru; Kawazu, Tetsu; Kobayashi, Yoshichika.

In: Photosynthesis Research, Vol. 81, No. 1, 01.07.2004, p. 67-76.

Research output: Contribution to journalArticle

Tsuyama, Michito ; Shibata, Masaru ; Kawazu, Tetsu ; Kobayashi, Yoshichika. / An analysis of the mechanism of the low-wave phenomenon of chlorophyll fluorescence. In: Photosynthesis Research. 2004 ; Vol. 81, No. 1. pp. 67-76.
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