An antigen conjugated SPG carrier for imunostimulatory CpG DNAs

Naohiko Shimada, Kazuo Sakurai, Yoichi Takeda, Seiji Shinkai, Ken Ishii

Research output: Contribution to conferencePaper

Abstract

CpG DNAs are known as an immune adjuvant to effectively induce Thl response. If CpG DNAs can induce Thl immune response coupled with a particular antigen, this would be more advantageous for anti-allergy therapy. We have reported that schizophyllan (SPG) can be used as a CpG DNA carrier. This study presents synthesis of an antigen-conjugated SPG and the characterization of the conjugate. Ovalbumin was used as a model antigen. CD spectra and gel electrophoresis showed that the conjugate could form a complex with a CpG DNA. Furthermore, the complex of the conjugate and DNA was co-localized in the same vesicles, implying that OVA (antigen) and CpG DNA (adjuvant) were ingested to the cell at the same time.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

Antigens
DNA
Allergies
Electrophoresis
Gels

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Shimada, N., Sakurai, K., Takeda, Y., Shinkai, S., & Ishii, K. (2006). An antigen conjugated SPG carrier for imunostimulatory CpG DNAs. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

An antigen conjugated SPG carrier for imunostimulatory CpG DNAs. / Shimada, Naohiko; Sakurai, Kazuo; Takeda, Yoichi; Shinkai, Seiji; Ishii, Ken.

2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

Research output: Contribution to conferencePaper

Shimada, N, Sakurai, K, Takeda, Y, Shinkai, S & Ishii, K 2006, 'An antigen conjugated SPG carrier for imunostimulatory CpG DNAs', Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06 - 5/26/06.
Shimada N, Sakurai K, Takeda Y, Shinkai S, Ishii K. An antigen conjugated SPG carrier for imunostimulatory CpG DNAs. 2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.
Shimada, Naohiko ; Sakurai, Kazuo ; Takeda, Yoichi ; Shinkai, Seiji ; Ishii, Ken. / An antigen conjugated SPG carrier for imunostimulatory CpG DNAs. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.1 p.
@conference{23e3e50401e04b8b91fe9aed2126808e,
title = "An antigen conjugated SPG carrier for imunostimulatory CpG DNAs",
abstract = "CpG DNAs are known as an immune adjuvant to effectively induce Thl response. If CpG DNAs can induce Thl immune response coupled with a particular antigen, this would be more advantageous for anti-allergy therapy. We have reported that schizophyllan (SPG) can be used as a CpG DNA carrier. This study presents synthesis of an antigen-conjugated SPG and the characterization of the conjugate. Ovalbumin was used as a model antigen. CD spectra and gel electrophoresis showed that the conjugate could form a complex with a CpG DNA. Furthermore, the complex of the conjugate and DNA was co-localized in the same vesicles, implying that OVA (antigen) and CpG DNA (adjuvant) were ingested to the cell at the same time.",
author = "Naohiko Shimada and Kazuo Sakurai and Yoichi Takeda and Seiji Shinkai and Ken Ishii",
year = "2006",
month = "10",
day = "19",
language = "English",
note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

}

TY - CONF

T1 - An antigen conjugated SPG carrier for imunostimulatory CpG DNAs

AU - Shimada, Naohiko

AU - Sakurai, Kazuo

AU - Takeda, Yoichi

AU - Shinkai, Seiji

AU - Ishii, Ken

PY - 2006/10/19

Y1 - 2006/10/19

N2 - CpG DNAs are known as an immune adjuvant to effectively induce Thl response. If CpG DNAs can induce Thl immune response coupled with a particular antigen, this would be more advantageous for anti-allergy therapy. We have reported that schizophyllan (SPG) can be used as a CpG DNA carrier. This study presents synthesis of an antigen-conjugated SPG and the characterization of the conjugate. Ovalbumin was used as a model antigen. CD spectra and gel electrophoresis showed that the conjugate could form a complex with a CpG DNA. Furthermore, the complex of the conjugate and DNA was co-localized in the same vesicles, implying that OVA (antigen) and CpG DNA (adjuvant) were ingested to the cell at the same time.

AB - CpG DNAs are known as an immune adjuvant to effectively induce Thl response. If CpG DNAs can induce Thl immune response coupled with a particular antigen, this would be more advantageous for anti-allergy therapy. We have reported that schizophyllan (SPG) can be used as a CpG DNA carrier. This study presents synthesis of an antigen-conjugated SPG and the characterization of the conjugate. Ovalbumin was used as a model antigen. CD spectra and gel electrophoresis showed that the conjugate could form a complex with a CpG DNA. Furthermore, the complex of the conjugate and DNA was co-localized in the same vesicles, implying that OVA (antigen) and CpG DNA (adjuvant) were ingested to the cell at the same time.

UR - http://www.scopus.com/inward/record.url?scp=33749866040&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749866040&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749866040

ER -