An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase

Takashi Ueda, Noriyuki Matsuda, Toyoaki Anai, Hirokazu Tsukaya, Hirofumi Uchimiya, Akihiko Nakano

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The Arabidopsis Ara proteins belong to the Reb/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated e clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the Rab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdl1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.

Original languageEnglish
Pages (from-to)2079-2091
Number of pages13
JournalPlant Cell
Volume8
Issue number11
DOIs
Publication statusPublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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