Abstract
The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.
| Original language | English |
|---|---|
| Pages (from-to) | 69-75 |
| Number of pages | 7 |
| Journal | Biocontrol Science |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2004 |
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All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
- Public Health, Environmental and Occupational Health
Cite this
An attempt to identify Bacillus cereus by PCR. / Miyamoto, Takahisa; Kamikado, Hideaki; Sasaki, Chie; Sadakari, Kiyoko; Honjoh, Ken-ichi; Iio, Masayoshi.
In: Biocontrol Science, Vol. 9, No. 3, 09.2004, p. 69-75.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An attempt to identify Bacillus cereus by PCR
AU - Miyamoto, Takahisa
AU - Kamikado, Hideaki
AU - Sasaki, Chie
AU - Sadakari, Kiyoko
AU - Honjoh, Ken-ichi
AU - Iio, Masayoshi
PY - 2004/9
Y1 - 2004/9
N2 - The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.
AB - The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.
UR - http://www.scopus.com/inward/record.url?scp=7044270603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7044270603&partnerID=8YFLogxK
U2 - 10.4265/bio.9.69
DO - 10.4265/bio.9.69
M3 - Article
AN - SCOPUS:7044270603
VL - 9
SP - 69
EP - 75
JO - Biocontrol Science
JF - Biocontrol Science
SN - 1342-4815
IS - 3
ER -