An attempt to identify Bacillus cereus by PCR

Takahisa Miyamoto; Hideaki Kamikado; Chie Sasaki; Kiyoko Sadakari; Ken-ichi Honjoh; Masayoshi Iio

doi:10.4265/bio.9.69

An attempt to identify Bacillus cereus by PCR

Takahisa Miyamoto, Hideaki Kamikado, Chie Sasaki, Kiyoko Sadakari, Ken-ichi Honjoh, Masayoshi Iio

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.

Original language	English
Pages (from-to)	69-75
Number of pages	7
Journal	Biocontrol Science
Volume	9
Issue number	3
DOIs	https://doi.org/10.4265/bio.9.69
Publication status	Published - Sep 2004

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

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title = "An attempt to identify Bacillus cereus by PCR",

abstract = "The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.",

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AU - Miyamoto, Takahisa

AU - Kamikado, Hideaki

AU - Sasaki, Chie

AU - Sadakari, Kiyoko

AU - Honjoh, Ken-ichi

AU - Iio, Masayoshi

PY - 2004/9

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AB - The DNA band patterns generated by PCR using Primer 4 and template DNAs from various Bacillus strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in B. cereus revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to B. cereus. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 bp PCR product with DNAs from B. cereus type strain JCM 2152, 3 cereulide-positive B. cereus strains and 20 strains among 49 B. cereus isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of B. thuringiensis, other 11 Bacillus species and 12 different bacteria which were not Bacillus.

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