An autocatalytic expression system for regulated production of recombinant protein in mammalian cells

Yingpei Zhang, Yoshinori Katakura, Hideya Ohashi, Sanetaka Shirahata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalAnalytical Biochemistry
Volume252
Issue number2
DOIs
Publication statusPublished - Oct 15 1997

Fingerprint

Tetracycline
Recombinant Proteins
Genes
Cells
Signal systems
Trans-Activators
Gene Amplification
Gene expression
Clone Cells
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

An autocatalytic expression system for regulated production of recombinant protein in mammalian cells. / Zhang, Yingpei; Katakura, Yoshinori; Ohashi, Hideya; Shirahata, Sanetaka.

In: Analytical Biochemistry, Vol. 252, No. 2, 15.10.1997, p. 286-292.

Research output: Contribution to journalArticle

Zhang, Yingpei ; Katakura, Yoshinori ; Ohashi, Hideya ; Shirahata, Sanetaka. / An autocatalytic expression system for regulated production of recombinant protein in mammalian cells. In: Analytical Biochemistry. 1997 ; Vol. 252, No. 2. pp. 286-292.
@article{d8eb970453b84bf5b5b989efebb26a36,
title = "An autocatalytic expression system for regulated production of recombinant protein in mammalian cells",
abstract = "An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.",
author = "Yingpei Zhang and Yoshinori Katakura and Hideya Ohashi and Sanetaka Shirahata",
year = "1997",
month = "10",
day = "15",
doi = "10.1006/abio.1997.2325",
language = "English",
volume = "252",
pages = "286--292",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - An autocatalytic expression system for regulated production of recombinant protein in mammalian cells

AU - Zhang, Yingpei

AU - Katakura, Yoshinori

AU - Ohashi, Hideya

AU - Shirahata, Sanetaka

PY - 1997/10/15

Y1 - 1997/10/15

N2 - An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.

AB - An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.

UR - http://www.scopus.com/inward/record.url?scp=0031572844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031572844&partnerID=8YFLogxK

U2 - 10.1006/abio.1997.2325

DO - 10.1006/abio.1997.2325

M3 - Article

VL - 252

SP - 286

EP - 292

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -