An autocatalytic expression system for regulated production of recombinant protein in mammalian cells

Yingpei Zhang, Yoshinori Katakura, Hideya Ohashi, Sanetaka Shirahata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalAnalytical Biochemistry
Volume252
Issue number2
DOIs
Publication statusPublished - Oct 15 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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