An ecofriendly green liquid chromatographic method for simultaneous determination of nicotinamide and clindamycin phosphate in pharmaceutical gel for acne treatment

Fawzia Ibrahim, Asmaa Kamal El-Deen, Samah Abo El Abass, Kuniyoshi Shimizu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new green micellar liquid chromatographic method was developed and validated for the quantitative estimation of nicotinamide (NICO) and clindamycin phosphate (CLD) in bulk and pharmaceutical gel formulation. The analytes are well resolved in less than 6.0 minutes using micellar mobile phase consisting of 0.10M sodium dodecyl sulfate (SDS), 0.3% triethylamine, and 10% 2-propanol in 0.02M orthophosphoric acid at pH 3.0, running through an Eclipse XDB-C8 column (150 mm × 4.6 mm, 5 μm particle size) with flow rate 1.0 mL/min. The effluent was monitored with diode array detection at 210 nm. The retention times of NICO and CLD were 3.8 minutes and 5.6 minutes, respectively. The method was validated according to the International Conference on Harmonisation (ICH) guidelines in terms of linearity, limit of detection, limit of quantification, accuracy, precision, robustness, and specificity to prove its reliability. Linear correlation was achieved by plotting the peak area of each drug against its concentration. It was found to be rectilinear in the ranges of 1.0–40.0 μg/mL and 0.5–15.0 μg/mL with limits of detection of 0.06 μg/mL and 0.03 μg/mL and limits of quantification of 0.19 μg/mL and 0.09 μg/mL for NICO and CLD, respectively. The method was successfully implemented for the simultaneous determination of the analytes in their bulk powder and combined gel formulation with high % recoveries. The ease of sample treatment facilitates and greatly expedites the treatment with reduced cost and improved accuracy of the procedure.

Original languageEnglish
Pages (from-to)741-747
Number of pages7
JournalJournal of Food and Drug Analysis
Volume25
Issue number3
DOIs
Publication statusPublished - Jul 2017

Fingerprint

acne
clindamycin
nicotinamide
Niacinamide
Acne Vulgaris
detection limit
Gels
gels
phosphates
drugs
liquids
Pharmaceutical Preparations
triethylamine
Limit of Detection
isopropyl alcohol
sodium dodecyl sulfate
Drug Compounding
effluents
2-Propanol
particle size

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

Cite this

An ecofriendly green liquid chromatographic method for simultaneous determination of nicotinamide and clindamycin phosphate in pharmaceutical gel for acne treatment. / Ibrahim, Fawzia; El-Deen, Asmaa Kamal; El Abass, Samah Abo; Shimizu, Kuniyoshi.

In: Journal of Food and Drug Analysis, Vol. 25, No. 3, 07.2017, p. 741-747.

Research output: Contribution to journalArticle

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