An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

Kazumitsu Onizuka, Takamasa Nishioka, Zhichun Li, Daichi Jitsuzaki, Yosuke Taniguchi, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

O6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.

Original languageEnglish
Pages (from-to)3969-3971
Number of pages3
JournalChemical Communications
Volume48
Issue number33
DOIs
Publication statusPublished - Mar 26 2012

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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