An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Naoto Shirahata, Atsushi Hozumi, Yoshiko Miura, Kazukiyo Kobayashi, Yoshio Sakka, Tetsu Yonezawa

Research output: Contribution to journalConference article

9 Citations (Scopus)

Abstract

Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalThin Solid Films
Volume499
Issue number1-2
DOIs
Publication statusPublished - Mar 21 2006
EventProceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004) -
Duration: Dec 15 2004Dec 17 2004

Fingerprint

Silanes
carbohydrates
Silicon
Carbohydrates
silanes
phosphates
Buffers
Phosphates
buffers
proteins
Proteins
Lectins
Adsorption
adsorption
silicon
matrices
lactose
Photomasks
photomasks
Lactose

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon. / Shirahata, Naoto; Hozumi, Atsushi; Miura, Yoshiko; Kobayashi, Kazukiyo; Sakka, Yoshio; Yonezawa, Tetsu.

In: Thin Solid Films, Vol. 499, No. 1-2, 21.03.2006, p. 213-218.

Research output: Contribution to journalConference article

Shirahata, Naoto ; Hozumi, Atsushi ; Miura, Yoshiko ; Kobayashi, Kazukiyo ; Sakka, Yoshio ; Yonezawa, Tetsu. / An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon. In: Thin Solid Films. 2006 ; Vol. 499, No. 1-2. pp. 213-218.
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