An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Naoto Shirahata; Atsushi Hozumi; Yoshiko Miura; Kazukiyo Kobayashi; Yoshio Sakka; Tetsu Yonezawa

doi:10.1016/j.tsf.2005.07.048

An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Naoto Shirahata, Atsushi Hozumi, Yoshiko Miura, Kazukiyo Kobayashi, Yoshio Sakka, Tetsu Yonezawa

Research output: Contribution to journal › Conference article › peer-review

9 Citations (Scopus)

Abstract

Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiO_x surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiO_x surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

Original language	English
Pages (from-to)	213-218
Number of pages	6
Journal	Thin Solid Films
Volume	499
Issue number	1-2
DOIs	https://doi.org/10.1016/j.tsf.2005.07.048
Publication status	Published - Mar 21 2006
Externally published	Yes
Event	Proceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004) - Duration: Dec 15 2004 → Dec 17 2004

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Surfaces and Interfaces
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1016/j.tsf.2005.07.048

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title = "An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon",

abstract = "Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.",

author = "Naoto Shirahata and Atsushi Hozumi and Yoshiko Miura and Kazukiyo Kobayashi and Yoshio Sakka and Tetsu Yonezawa",

note = "Funding Information: This study was partly performed through Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.; Proceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004) ; Conference date: 15-12-2004 Through 17-12-2004",

year = "2006",

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doi = "10.1016/j.tsf.2005.07.048",

language = "English",

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journal = "Thin Solid Films",

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T1 - An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

AU - Shirahata, Naoto

AU - Hozumi, Atsushi

AU - Miura, Yoshiko

AU - Kobayashi, Kazukiyo

AU - Sakka, Yoshio

AU - Yonezawa, Tetsu

N1 - Funding Information: This study was partly performed through Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.

PY - 2006/3/21

Y1 - 2006/3/21

N2 - Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

AB - Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

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SN - 0040-6090

VL - 499

SP - 213

EP - 218

JO - Thin Solid Films

JF - Thin Solid Films

IS - 1-2

T2 - Proceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004)

Y2 - 15 December 2004 through 17 December 2004

ER -

An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Abstract

All Science Journal Classification (ASJC) codes

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