An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Naoto Shirahata, Atsushi Hozumi, Yoshiko Miura, Kazukiyo Kobayashi, Yoshio Sakka, Tetsu Yonezawa

Research output: Contribution to journalConference article

9 Citations (Scopus)

Abstract

Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalThin Solid Films
Volume499
Issue number1-2
DOIs
Publication statusPublished - Mar 21 2006
Externally publishedYes
EventProceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004) -
Duration: Dec 15 2004Dec 17 2004

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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