An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor

Hiroki Ishikuro, Eiji Nitasaka, Motoyasu Otani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We established an efficient plant regeneration system to form embryogenic calli in common morning glory (Ipomoea purpurea) and blue morning glory (I. tricolor). Immature embryos of both morning glories cultured on media containing 1 mg l-1 4-fluorophenoxyacetic acid (4FA) and 6% sucrose formed many embryogenic calli. The frequency of embryogenic callus formation was highest in I. purpurea strain Q74 (42.5%) and in I. tricolor cultivar 'Flying Saucers' (36.7%). Embryogenic callus formation differed with the genotypes in both morning glories. Numerous somatic embryos were formed from the embryogenic calli when the calli were transferred onto plant growth regulator-free medium.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalPlant Biotechnology
Volume31
Issue number2
DOIs
Publication statusPublished - 2014

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Ipomoea tricolor
Ipomoea purpurea
Ipomoea
embryo culture
callus formation
Bony Callus
somatic embryogenesis
Embryonic Development
Regeneration
callus
Embryonic Structures
immatures
somatic embryos
plant growth substances
embryo (plant)
Plant Growth Regulators
flight
sucrose
Sucrose
genotype

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

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abstract = "We established an efficient plant regeneration system to form embryogenic calli in common morning glory (Ipomoea purpurea) and blue morning glory (I. tricolor). Immature embryos of both morning glories cultured on media containing 1 mg l-1 4-fluorophenoxyacetic acid (4FA) and 6{\%} sucrose formed many embryogenic calli. The frequency of embryogenic callus formation was highest in I. purpurea strain Q74 (42.5{\%}) and in I. tricolor cultivar 'Flying Saucers' (36.7{\%}). Embryogenic callus formation differed with the genotypes in both morning glories. Numerous somatic embryos were formed from the embryogenic calli when the calli were transferred onto plant growth regulator-free medium.",
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