An electrochemical device for the assay of the interaction between a dioxin receptor and its various ligands

Masaharu Murata, Hatsumi Gonda, Kentaro Yano, Shinichiro Kuroki, Tatsuo Suzutani, Yoshiki Katayama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxic and biological effects of a variety of chemicals. Although a significant amount of information is available with respect to the planar aromatic hydrocarbon AhR ligands, information on the actual spectrum of chemical structures that can bind to and activate the AhR is insufficient. In order to determine the binding affinities of chemicals to the human AhR (hAhR), we constructed an electrochemical system which carries the hAhR ligand-binding domain on the electrode surface. The recombinant hAhR ligand-binding domain that was expressed in Escherichia coli using a T7 expression system was immobilized on a gold electrode. The specificity of this biosensor based on a ligand-receptor interaction was comparable to other in vitro screening methods. The receptor-modified electrode can rapidly detect the binding of ligands to hAhR. The electrochemical measurement can be carried out within just 5 min. This electrochemical screening system is rapid, low in cost, and adaptable to high-throughput applications without sacrificing either sensitivity or selectivity.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 5 2004

Fingerprint

Aryl Hydrocarbon Receptors
Assays
Ligands
Equipment and Supplies
Electrodes
Screening
Aromatic Hydrocarbons
Poisons
Biosensing Techniques
Biosensors
Gold
Escherichia coli
Transcription Factors
Throughput
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

An electrochemical device for the assay of the interaction between a dioxin receptor and its various ligands. / Murata, Masaharu; Gonda, Hatsumi; Yano, Kentaro; Kuroki, Shinichiro; Suzutani, Tatsuo; Katayama, Yoshiki.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 14, No. 1, 05.01.2004, p. 137-141.

Research output: Contribution to journalArticle

Murata, Masaharu ; Gonda, Hatsumi ; Yano, Kentaro ; Kuroki, Shinichiro ; Suzutani, Tatsuo ; Katayama, Yoshiki. / An electrochemical device for the assay of the interaction between a dioxin receptor and its various ligands. In: Bioorganic and Medicinal Chemistry Letters. 2004 ; Vol. 14, No. 1. pp. 137-141.
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