An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I

Watoo Phrompittayarat, Waraporn Putalun, Hiroyuki Tanaka, Sakchai Wittaya-Areekul, Kanchalee Jetiyanon, Kornkanok Ingkaninan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5 ng mL-1 of bacopaside I and the limit of detection was 0.1 ng mL-1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalAnalytica Chimica Acta
Volume584
Issue number1
DOIs
Publication statusPublished - Feb 12 2007

Fingerprint

Glycosides
antibody
Assays
assay
enzyme
Antibodies
Enzymes
Saponins
medicinal plant
Bacopa
Ayurvedic Medicine
medicine
serum
Medicinal Plants
Bovine Serum Albumin
Medicine
Limit of Detection
bacopaside I
Rabbits
Data storage equipment

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Phrompittayarat, W., Putalun, W., Tanaka, H., Wittaya-Areekul, S., Jetiyanon, K., & Ingkaninan, K. (2007). An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I. Analytica Chimica Acta, 584(1), 1-6. https://doi.org/10.1016/j.aca.2006.11.017

An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I. / Phrompittayarat, Watoo; Putalun, Waraporn; Tanaka, Hiroyuki; Wittaya-Areekul, Sakchai; Jetiyanon, Kanchalee; Ingkaninan, Kornkanok.

In: Analytica Chimica Acta, Vol. 584, No. 1, 12.02.2007, p. 1-6.

Research output: Contribution to journalArticle

Phrompittayarat, W, Putalun, W, Tanaka, H, Wittaya-Areekul, S, Jetiyanon, K & Ingkaninan, K 2007, 'An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I', Analytica Chimica Acta, vol. 584, no. 1, pp. 1-6. https://doi.org/10.1016/j.aca.2006.11.017
Phrompittayarat W, Putalun W, Tanaka H, Wittaya-Areekul S, Jetiyanon K, Ingkaninan K. An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I. Analytica Chimica Acta. 2007 Feb 12;584(1):1-6. https://doi.org/10.1016/j.aca.2006.11.017
Phrompittayarat, Watoo ; Putalun, Waraporn ; Tanaka, Hiroyuki ; Wittaya-Areekul, Sakchai ; Jetiyanon, Kanchalee ; Ingkaninan, Kornkanok. / An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I. In: Analytica Chimica Acta. 2007 ; Vol. 584, No. 1. pp. 1-6.
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