An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I

Watoo Phrompittayarat, Waraporn Putalun, Hiroyuki Tanaka, Sakchai Wittaya-Areekul, Kanchalee Jetiyanon, Kornkanok Ingkaninan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5 ng mL-1 of bacopaside I and the limit of detection was 0.1 ng mL-1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalAnalytica Chimica Acta
Volume584
Issue number1
DOIs
Publication statusPublished - Feb 12 2007

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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