An enzyme-linked immunosorbent assay for aconitine-type alkaloids using an anti-aconitine monoclonal antibody

Katsumi Kido, Kyoko Edakuni, Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

3-Succinylaconitine was conjugated with bovine serum albumin (BSA) for use as an immunogen for the preparation of a monoclonal antibody (MAb) against aconitine (Aco). Splenocytes from mice immunized with the Aco-BSA conjugate were fused with an aminopterin-sensitive mouse myeloma cell line, P3-X63-Ag8-653, and a hybridoma secreting a MAb against Aco was successfully obtained. The MAb cross-reacted with mesaconitine, hypaconitine and jesaconitine, which are Aco-type alkaloids, but not with any other compounds examined. The full measurement range of an enzyme-linked immunosorbent assay (ELISA) developed using the new MAb extended from 100 ng mL-1 to 1.5 μg mL-1 of Aco. The concentrations of Aco-type alkaloids in various Aconiti radixes assayed using the new ELISA method showed good agreement with previous reports.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalAnalytica Chimica Acta
Volume616
Issue number1
DOIs
Publication statusPublished - May 26 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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