An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki; Nobumi Takamatsu; Toshihiro Ansai; Yoshihisa Yamashita; Tadamichi Takehara; Toshihiko Koga

doi:10.1016/0167-7012(95)91417-D

An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki, Nobumi Takamatsu, Toshihiro Ansai, Yoshihisa Yamashita, Tadamichi Takehara, Toshihiko Koga

Oral health, growth and development

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

Original language	English
Pages (from-to)	181-192
Number of pages	12
Journal	Journal of Microbiological Methods
Volume	21
Issue number	2
DOIs	https://doi.org/10.1016/0167-7012(95)91417-D
Publication status	Published - Feb 1995

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/0167-7012(95)91417-D

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An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. / Kawasaki, Masahito; Takamatsu, Nobumi; Ansai, Toshihiro; Yamashita, Yoshihisa; Takehara, Tadamichi; Koga, Toshihiko.

In: Journal of Microbiological Methods, Vol. 21, No. 2, 02.1995, p. 181-192.

Research output: Contribution to journal › Article › peer-review

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