An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki; Nobumi Takamatsu; Toshihiro Ansai; Yoshihisa Yamashita; Tadamichi Takehara; Toshihiko Koga

doi:10.1016/0167-7012(95)91417-D

An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki, Nobumi Takamatsu, Toshihiro Ansai, Yoshihisa Yamashita, Tadamichi Takehara, Toshihiko Koga

Oral health, growth and development

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Abstract

An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

Original language	English
Pages (from-to)	181-192
Number of pages	12
Journal	Journal of Microbiological Methods
Volume	21
Issue number	2
DOIs	https://doi.org/10.1016/0167-7012(95)91417-D
Publication status	Published - Feb 1995

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/0167-7012(95)91417-D

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title = "An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans",

abstract = "An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.",

author = "Masahito Kawasaki and Nobumi Takamatsu and Toshihiro Ansai and Yoshihisa Yamashita and Tadamichi Takehara and Toshihiko Koga",

note = "Funding Information: This work was supported in part by a grant-in-aid for scientific research (05454563f)r om the Ministry of Education, Science,a nd Culture of Japan and a researchg rant from the Japan Foundation for Aging and Health.",

year = "1995",

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doi = "10.1016/0167-7012(95)91417-D",

language = "English",

volume = "21",

pages = "181--192",

journal = "Journal of Microbiological Methods",

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T1 - An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

AU - Kawasaki, Masahito

AU - Takamatsu, Nobumi

AU - Ansai, Toshihiro

AU - Yamashita, Yoshihisa

AU - Takehara, Tadamichi

AU - Koga, Toshihiko

N1 - Funding Information: This work was supported in part by a grant-in-aid for scientific research (05454563f)r om the Ministry of Education, Science,a nd Culture of Japan and a researchg rant from the Japan Foundation for Aging and Health.

PY - 1995/2

Y1 - 1995/2

N2 - An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

AB - An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

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SN - 0167-7012

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An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Abstract

All Science Journal Classification (ASJC) codes

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