Abstract
An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.
| Original language | English |
|---|---|
| Pages (from-to) | 181-192 |
| Number of pages | 12 |
| Journal | Journal of Microbiological Methods |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1995 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Microbiology
- Microbiology (medical)
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. / Kawasaki, Masahito; Takamatsu, Nobumi; Ansai, Toshihiro; Yamashita, Yoshihisa; Takehara, Tadamichi; Koga, Toshihiko.
In: Journal of Microbiological Methods, Vol. 21, No. 2, 01.01.1995, p. 181-192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans
AU - Kawasaki, Masahito
AU - Takamatsu, Nobumi
AU - Ansai, Toshihiro
AU - Yamashita, Yoshihisa
AU - Takehara, Tadamichi
AU - Koga, Toshihiko
PY - 1995/1/1
Y1 - 1995/1/1
N2 - An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.
AB - An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.
UR - http://www.scopus.com/inward/record.url?scp=0028931957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028931957&partnerID=8YFLogxK
U2 - 10.1016/0167-7012(95)91417-D
DO - 10.1016/0167-7012(95)91417-D
M3 - Article
AN - SCOPUS:0028931957
VL - 21
SP - 181
EP - 192
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
IS - 2
ER -