An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki, Nobumi Takamatsu, Toshihiro Ansai, Yoshihisa Yamashita, Tadamichi Takehara, Toshihiko Koga

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

Original languageEnglish
Pages (from-to)181-192
Number of pages12
JournalJournal of Microbiological Methods
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 1995

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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