An Escherichia coli K12 mutant carrying altered ribosomal protein (S10)

Michihiko Kuwano, Hatsumi Taniguchi, Mayumi Ono, Hideya Endo, Yoshinari Ohnishi

Research output: Contribution to journalArticle

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Abstract

An Escherichia coli mutant (JE14373) carrying decreased stability of stable RNA species was found to have altered electrophoretic mobility of a 30S ribosomal protein (S10). Recombinants covering str gene (76 min on E. coli linkage map by Bachmann, Low and Taylor, 1976 (ref. 1)) obtained from a cross of CSH64 × JE14373, restored normal S10 protein. The size analysis of RNAs labeled for 15 min with [3H]uridine showed 50 to 60 % decrease of 16S RNA in this mutant strain, but almost no decrease of 23S RNA at 10 or 40 min after addition of rifampicin. On the other hand, no change was observed in the stability of both rRNA pieces in its parental PA3092 strain even at 40 min after addition of rifampicin.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume75
Issue number1
DOIs
Publication statusPublished - Mar 7 1977

Fingerprint

Escherichia coli K12
Escherichia coli
RNA
Rifampin
S 10
Uridine
RNA Stability
Electrophoretic mobility
Genes
ribosomal protein S10
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

An Escherichia coli K12 mutant carrying altered ribosomal protein (S10). / Kuwano, Michihiko; Taniguchi, Hatsumi; Ono, Mayumi; Endo, Hideya; Ohnishi, Yoshinari.

In: Biochemical and Biophysical Research Communications, Vol. 75, No. 1, 07.03.1977, p. 156-162.

Research output: Contribution to journalArticle

Kuwano, Michihiko ; Taniguchi, Hatsumi ; Ono, Mayumi ; Endo, Hideya ; Ohnishi, Yoshinari. / An Escherichia coli K12 mutant carrying altered ribosomal protein (S10). In: Biochemical and Biophysical Research Communications. 1977 ; Vol. 75, No. 1. pp. 156-162.
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