An ESR contrast agent is transported to rat liver through organic anion transporter

Kazuhiro Ichikawa, Yasuko Sato, Hiroshi Kondo, Hideo Utsumi

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Carboxy PROXYL is a useful extracellular paramagnetic contrast reagent in electron spin resonance (ESR) and magnetic resonance imaging (MRI). Active transfer of the probe was investigated using an in situ liver model in rats. Carboxy PROXYL, a nitroxyl spin probe, was perfused into in situ liver perfusion system from Wistar rats. Concentration of nitroxyl form of the spin probe in effluent increased gradually after introducing perfusate with the spin probe and reached a plateau. The disappearance of Carboxy PROXYL from the perfusate was 40%, which could not be explained with its partition coefficient. Administration of non-selective inhibitors of organic anion transporters, p -aminohippuric acid and penicillin G, inhibited competitively and in a dose dependent manner the transfer of Carboxy PROXYL into rat liver in situ, resulting in increases of Carboxy PROXYL in the effluent. The results demonstrate that there is an active transfer system of an ESR contrast reagent into in situ rat liver through organic anion transporters.

    Original languageEnglish
    Pages (from-to)403-408
    Number of pages6
    JournalFree Radical Research
    Volume40
    Issue number4
    DOIs
    Publication statusPublished - Apr 1 2006

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    Organic Anion Transporters
    Electron Spin Resonance Spectroscopy
    Liver
    Contrast Media
    Paramagnetic resonance
    Rats
    Effluents
    p-Aminohippuric Acid
    Penicillin G
    Magnetic resonance
    Wistar Rats
    Perfusion
    Magnetic Resonance Imaging
    Imaging techniques
    nitroxyl

    All Science Journal Classification (ASJC) codes

    • Biochemistry

    Cite this

    An ESR contrast agent is transported to rat liver through organic anion transporter. / Ichikawa, Kazuhiro; Sato, Yasuko; Kondo, Hiroshi; Utsumi, Hideo.

    In: Free Radical Research, Vol. 40, No. 4, 01.04.2006, p. 403-408.

    Research output: Contribution to journalArticle

    Ichikawa, Kazuhiro ; Sato, Yasuko ; Kondo, Hiroshi ; Utsumi, Hideo. / An ESR contrast agent is transported to rat liver through organic anion transporter. In: Free Radical Research. 2006 ; Vol. 40, No. 4. pp. 403-408.
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