An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody

Tharita Kitisripanya, Witcha Sermpradit, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

Abstract

There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.

Original languageEnglish
Article numbere4718
JournalBiomedical Chromatography
Volume34
Issue number2
DOIs
Publication statusPublished - Feb 1 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody'. Together they form a unique fingerprint.

  • Cite this