An evaluation method of bacterial flora in contaminated soils - For the purpose of a countermeasure against H2S gas production

Hatsumi Taniguchi, Kazumasa Fukuda, Wan Yan, Mitsuo Hinoue, Kazuki Yamauchi, Tsuyoshi Ichihara, Kouhei Mizuno, Sumiyo Ishimatsu, Nobuyuki Sera, Mitsuhiro Hamasaki, Kouji Takahashi, Kazumi Horikawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, spore-forming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium.

Original languageEnglish
Pages (from-to)349-367
Number of pages19
JournalJournal of UOEH
Issue number3
Publication statusPublished - Sep 1 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

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