An evaluation of the utility of the hepatic differentiation method using hollow fiber/organoid culture for the development of a hybrid artificial liver device

Naoki Amimoto, Hiroshi Mizumoto, Kohji Nakazawa, Hiroyuki Ijima, Kazumori Funatsu, Toshihisa Kajiwara

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3 Citations (Scopus)

Abstract

To put the hybrid artificial liver (HAL) using cultured hepatocytes into practical use, it is necessary to develop a high-performance artificial liver device. We developed a novel hollow fiber (HF)/organoid culture method to induce the differentiation of pluripotent stem cells into hepatocytes. In this study, we compared the results of the hepatic differentiation using the HF/organoid culture with those using monolayer culture to evaluate its utility as a hepatic differentiation method. In both cell cultures, ES cells showed high proliferative activity immediately after cell seeding. The up-regulation of hepatocyte-specific markers such as albumin (ALB), carbamoyl phosphate synthetase 1 (CPS-1) and tryptophan 2,. 3-dioxygenase (TDO) were observed as the culture progressed, and the expression of liver-specific functions such as the removal of ammonia and albumin secretion were detected after about 2 weeks of the hepatic differentiation induction in the HF/organoid culture. However, the results were not observed in the monolayer culture. In conclusion, the HF/organoid culture method has promise as an effective tool for the differentiation of ES cells into hepatocytes.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalBiochemical Engineering Journal
Volume56
Issue number1-2
DOIs
Publication statusPublished - Sep 15 2011

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Organoids
Artificial Liver
Liver
Hepatocytes
Equipment and Supplies
Fibers
Cell culture
Albumins
Monolayers
Tryptophan Synthase
Tryptophan Oxygenase
Carbamyl Phosphate
Pluripotent Stem Cells
Ligases
Stem cells
Ammonia
Tryptophan
Cell Differentiation
Phosphates
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

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abstract = "To put the hybrid artificial liver (HAL) using cultured hepatocytes into practical use, it is necessary to develop a high-performance artificial liver device. We developed a novel hollow fiber (HF)/organoid culture method to induce the differentiation of pluripotent stem cells into hepatocytes. In this study, we compared the results of the hepatic differentiation using the HF/organoid culture with those using monolayer culture to evaluate its utility as a hepatic differentiation method. In both cell cultures, ES cells showed high proliferative activity immediately after cell seeding. The up-regulation of hepatocyte-specific markers such as albumin (ALB), carbamoyl phosphate synthetase 1 (CPS-1) and tryptophan 2,. 3-dioxygenase (TDO) were observed as the culture progressed, and the expression of liver-specific functions such as the removal of ammonia and albumin secretion were detected after about 2 weeks of the hepatic differentiation induction in the HF/organoid culture. However, the results were not observed in the monolayer culture. In conclusion, the HF/organoid culture method has promise as an effective tool for the differentiation of ES cells into hepatocytes.",
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AU - Amimoto, Naoki

AU - Mizumoto, Hiroshi

AU - Nakazawa, Kohji

AU - Ijima, Hiroyuki

AU - Funatsu, Kazumori

AU - Kajiwara, Toshihisa

PY - 2011/9/15

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N2 - To put the hybrid artificial liver (HAL) using cultured hepatocytes into practical use, it is necessary to develop a high-performance artificial liver device. We developed a novel hollow fiber (HF)/organoid culture method to induce the differentiation of pluripotent stem cells into hepatocytes. In this study, we compared the results of the hepatic differentiation using the HF/organoid culture with those using monolayer culture to evaluate its utility as a hepatic differentiation method. In both cell cultures, ES cells showed high proliferative activity immediately after cell seeding. The up-regulation of hepatocyte-specific markers such as albumin (ALB), carbamoyl phosphate synthetase 1 (CPS-1) and tryptophan 2,. 3-dioxygenase (TDO) were observed as the culture progressed, and the expression of liver-specific functions such as the removal of ammonia and albumin secretion were detected after about 2 weeks of the hepatic differentiation induction in the HF/organoid culture. However, the results were not observed in the monolayer culture. In conclusion, the HF/organoid culture method has promise as an effective tool for the differentiation of ES cells into hepatocytes.

AB - To put the hybrid artificial liver (HAL) using cultured hepatocytes into practical use, it is necessary to develop a high-performance artificial liver device. We developed a novel hollow fiber (HF)/organoid culture method to induce the differentiation of pluripotent stem cells into hepatocytes. In this study, we compared the results of the hepatic differentiation using the HF/organoid culture with those using monolayer culture to evaluate its utility as a hepatic differentiation method. In both cell cultures, ES cells showed high proliferative activity immediately after cell seeding. The up-regulation of hepatocyte-specific markers such as albumin (ALB), carbamoyl phosphate synthetase 1 (CPS-1) and tryptophan 2,. 3-dioxygenase (TDO) were observed as the culture progressed, and the expression of liver-specific functions such as the removal of ammonia and albumin secretion were detected after about 2 weeks of the hepatic differentiation induction in the HF/organoid culture. However, the results were not observed in the monolayer culture. In conclusion, the HF/organoid culture method has promise as an effective tool for the differentiation of ES cells into hepatocytes.

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