An experimental study of inner ear injury in an animal model of eosinophilic otitis media

Atsushi Matsubara, Hisanori Nishizawa, Akira Kurose, Takashi Nakagawa, Junko Takahata, Akira Sasaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Conclusion: As the periods of intratympanic injection of ovalbumin (OVA) to the middle ear became longer, marked eosinophil infiltration in the perilymphatic space was observed. Moreover severe morphological damage of the organ of Corti was observed in the 28-day antigen-stimulation side. These results indicate that eosinophilic inflammation occurred in the inner ear and caused profound hearing loss. Objective: The purpose of the present study was to elucidate the inner ear damage in a new animal model of eosinophilic otitis media (EOM) which we recently constructed. Methods: We constructed the animal model of EOM by intraperitoneal and intratympanic injection of OVA. Infiltrating cells and the inner ear damage were examined by histological study. Results: In the inner ear, a few eosinophils were seen in the scala tympani of the organ of Corti and the dilation of capillaries of the stria vascularis was observed in the 7-day stimulation side. In the 14-day antigen stimulation side, some eosinophils and macrophages were seen in not only the scala tympani but also the scala vestibule. In the 28-day antigen-stimulation side, severe morphological damage of the organ of Corti and many eosinophils, red blood cells, and plasma cells infiltrating the perilymph were observed.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalActa Oto-Laryngologica
Volume134
Issue number3
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

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Otitis Media
Inner Ear
Eosinophils
Organ of Corti
Animal Models
Scala Tympani
Ovalbumin
Wounds and Injuries
Antigens
Stria Vascularis
Perilymph
Middle Ear
Plasma Cells
Intraperitoneal Injections
Hearing Loss
Dilatation
Erythrocytes
Macrophages
Inflammation
Intratympanic Injection

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

An experimental study of inner ear injury in an animal model of eosinophilic otitis media. / Matsubara, Atsushi; Nishizawa, Hisanori; Kurose, Akira; Nakagawa, Takashi; Takahata, Junko; Sasaki, Akira.

In: Acta Oto-Laryngologica, Vol. 134, No. 3, 01.03.2014, p. 227-232.

Research output: Contribution to journalArticle

Matsubara, Atsushi ; Nishizawa, Hisanori ; Kurose, Akira ; Nakagawa, Takashi ; Takahata, Junko ; Sasaki, Akira. / An experimental study of inner ear injury in an animal model of eosinophilic otitis media. In: Acta Oto-Laryngologica. 2014 ; Vol. 134, No. 3. pp. 227-232.
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