An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration

Akiko Mizokami, Da Guang Wang, Mitsuru Tanaka, Jing Gao, Hiroshi Takeuchi, Toshiro Matsui, Masato Hirata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Osteocalcin (OC) is a bone-derived hormone that regulates energy metabolism. OC exists in two forms, carboxylated (GlaOC) and uncaboxylated (GluOC), but only the latter appears to have an endocrine function. In this study, we prepared an extract containing both Gla- and GluOC from boiled pork bone using 0.2 M carbonate buffer at pH 9.5, and tested whether the extract had beneficial effects on improving metabolic parameters in obese mice. The extract equivalent of 1.2 μg of GluOC/mouse was orally administrated to C57BL/6 female mice fed a high-fat, high-sucrose diet. Daily oral administration of the extract for four weeks decreased blood glucose levels and promoted glucose tolerance as well as insulin sensitivity. Our study shows for the first time that boiled pork bones are a source material for osteocalcin in the large-scale production of supplements designed to improve glucose metabolism.

Original languageEnglish
Pages (from-to)2176-2183
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number11
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Osteocalcin
Metabolism
Oral Administration
Bone
Bone and Bones
Glucose
Obese Mice
Carbonates
High Fat Diet
Nutrition
Energy Metabolism
Sucrose
Insulin Resistance
Blood Glucose
Buffers
Fats
Hormones
Insulin
Red Meat

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration. / Mizokami, Akiko; Wang, Da Guang; Tanaka, Mitsuru; Gao, Jing; Takeuchi, Hiroshi; Matsui, Toshiro; Hirata, Masato.

In: Bioscience, Biotechnology and Biochemistry, Vol. 80, No. 11, 01.01.2016, p. 2176-2183.

Research output: Contribution to journalArticle

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