An HLA-binding-motif-aided peptide epitope library: A novel library design for the screening of HLA-DR4-restricted antigenic peptides recognized by CD4+T cells

Takeshi Tana, Nobuhiro Kamikawaji, Christopher J. Savoie, Tohru Sudo, Yurika Kinoshita, Takehiko Sasazuki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Susceptibility to a series of autoimmune diseases is strongly associated with particular HLA class II alleles. Identification of T cell clones and antigenic epitopes bound by HLA class II molecules involved in autoimmune diseases is critical to understanding the etiology of these HLA class II- associated diseases. However, establishment of T cell clones in autoimmune diseases is difficult because the antigenic peptides are unknown. Peptide library methods which include all possible peptide sequences offer a potentially powerful tool for the detection of cross-reactive antigenic peptides recognized by T cells. Here, we reduced the number of peptides per mixture by utilizing the known binding motifs of peptides for the HLA- DRB1*0405 molecule and evaluated the effectiveness of this library design. Each library mixture evoked a strong proliferative response in the unprimed peripheral blood lymphocytes (PBL) from HLA-DRB1*0405-positive donors but little or no response in the PBL from HLA-DRB1*0405-negative donors. The library also detected antigenic peptides that activated three antigen- specific T cell lines restricted by HLA-DRB1*0405, with different specificities. The motif-based approach thus presents a powerful method for monitoring T cells in large, heterogeneous T cell populations and is useful for the identification of the mimic peptide epitopes of T cell lines and clones.

Original languageEnglish
Pages (from-to)14-21
Number of pages8
JournalJournal of Human Genetics
Volume43
Issue number1
DOIs
Publication statusPublished - Jun 13 1998

Fingerprint

HLA-DR4 Antigen
Peptide Library
Libraries
Epitopes
T-Lymphocytes
Peptides
Autoimmune Diseases
Peptide T
Clone Cells
Lymphocytes
Cell Line
Alleles
Antigens
HLA-DRB1*04:05 antigen

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

An HLA-binding-motif-aided peptide epitope library : A novel library design for the screening of HLA-DR4-restricted antigenic peptides recognized by CD4+T cells. / Tana, Takeshi; Kamikawaji, Nobuhiro; Savoie, Christopher J.; Sudo, Tohru; Kinoshita, Yurika; Sasazuki, Takehiko.

In: Journal of Human Genetics, Vol. 43, No. 1, 13.06.1998, p. 14-21.

Research output: Contribution to journalArticle

Tana, Takeshi ; Kamikawaji, Nobuhiro ; Savoie, Christopher J. ; Sudo, Tohru ; Kinoshita, Yurika ; Sasazuki, Takehiko. / An HLA-binding-motif-aided peptide epitope library : A novel library design for the screening of HLA-DR4-restricted antigenic peptides recognized by CD4+T cells. In: Journal of Human Genetics. 1998 ; Vol. 43, No. 1. pp. 14-21.
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