TY - JOUR
T1 - An IκB-β COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress
AU - Lao, Qizong
AU - Kuge, Osamu
AU - Fukamachi, Toshihiko
AU - Kakegawa, Tomohito
AU - Saito, Hiromi
AU - Nishijima, Masahiro
AU - Kobayashi, Hiroshi
PY - 2005/4/1
Y1 - 2005/4/1
N2 - CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
AB - CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
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U2 - 10.1002/jcp.20221
DO - 10.1002/jcp.20221
M3 - Article
C2 - 15484229
AN - SCOPUS:14744278599
VL - 203
SP - 186
EP - 192
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 1
ER -