Abstract
CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
| Original language | English |
|---|---|
| Pages (from-to) | 186-192 |
| Number of pages | 7 |
| Journal | Journal of cellular physiology |
| Volume | 203 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 1 2005 |
All Science Journal Classification (ASJC) codes
- Physiology
- Clinical Biochemistry
- Cell Biology
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Lao, Q., Kuge, O., Fukamachi, T., Kakegawa, T., Saito, H., Nishijima, M., & Kobayashi, H. (2005). An IκB-β COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress. Journal of cellular physiology, 203(1), 186-192. https://doi.org/10.1002/jcp.20221