Abstract
CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
| Original language | English |
|---|---|
| Pages (from-to) | 186-192 |
| Number of pages | 7 |
| Journal | Journal of cellular physiology |
| Volume | 203 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 1 2005 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Clinical Biochemistry
- Cell Biology
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An IκB-β COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress. / Lao, Qizong; Kuge, Osamu; Fukamachi, Toshihiko; Kakegawa, Tomohito; Saito, Hiromi; Nishijima, Masahiro; Kobayashi, Hiroshi.
In: Journal of cellular physiology, Vol. 203, No. 1, 01.04.2005, p. 186-192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An IκB-β COOH terminal region protein is essential for the proliferation of CHO cells under acidic stress
AU - Lao, Qizong
AU - Kuge, Osamu
AU - Fukamachi, Toshihiko
AU - Kakegawa, Tomohito
AU - Saito, Hiromi
AU - Nishijima, Masahiro
AU - Kobayashi, Hiroshi
PY - 2005/4/1
Y1 - 2005/4/1
N2 - CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
AB - CHO-K1 cells were able to proliferate and maintain pHi homeostasis at pH 6.3. A novel acidic sensitive mutant, AS-5B, which proliferated at pH 7.4 but failed to either proliferate or maintain pHi homeostasis at pH 6.3, was derived from CHO-K1 using a replica method. The acidic-sensitivity of AS-5B was not due to deficiencies in sodium proton exchangers, HCO3- (co)transporters or H+-ATPases. A cDNA clone encoding a COOH terminal region of IκB-β conferred partial acidic-resistance on AS-5B, and the encoded protein was present in CHO-K1, but was nearly absent from AS-5B. Our data demonstrated that the expression of this small protein was essential for the proliferation of CHO cells under acidic stress.
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UR - http://www.scopus.com/inward/citedby.url?scp=14744278599&partnerID=8YFLogxK
U2 - 10.1002/jcp.20221
DO - 10.1002/jcp.20221
M3 - Article
C2 - 15484229
AN - SCOPUS:14744278599
VL - 203
SP - 186
EP - 192
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 1
ER -