An immunoassay method for the pharmacokinetics of 5-fluorouracil in patients with gastric cancer administered adjuvant chemotherapy

Hideo Matsumoto, Masaharu Higashida, Hisako Kubota, Haruaki Murakami, Kosuke Tsutsumi, Hiroshi Nakashima, Yasuo Oka, Hideo Okumura, Masafumi Nakamura, Toshihiro Hirai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Conventional gas chromatography-mass spectrometry (GC-MS) was compared with a new immunoassay method for measuring plasma (5-FU) concentrations in adjuvant chemotherapy with TS-1 for patients with gastric cancer. TS-1 was administered orally to patients after gastrectomy. Blood samples for pharmacokinetic analysis were collected on the seventh day of treatment. The mean area under the time concentration curve (AUC)0-8, half-life (t1/2), and maximum drug concentration (Cmax) obtained by the two methods were as follows: GC-MS, 847.9 μg/ml/hr, 2.76 h, and 186.6 ng/ml; and immunoassay, 1311.2 μg/ml/hr, 3.5 h, and 259.8 ng/ml. Significant correlations were observed for AUC0-8(p=0.0001), Cmax (p=0.0007), and changes in the 5-FU concentration in blood over time (p=0.018) for the two methods. Compared with the conventional GC-MS method, the new immunoassay method provides similar results, but is simpler and results can be obtained earlier. This method will be useful for monitoring the 5-FU concentration in serum from patients with gastric cancer receiving TS-1.

Original languageEnglish
Pages (from-to)5111-5114
Number of pages4
JournalAnticancer research
Volume32
Issue number11
Publication statusPublished - Nov 1 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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