An immunohistochemical analysis of PD-L1 protein expression in surgically resected small cell lung cancer using different antibodies and criteria

Kazuki Takada, Gouji Toyokawa, Tatsuro Okamoto, Takaki Akamine, Shinkichi Takamori, Masakazu Katsura, Takatoshi Fujishita, Fumihiro Shoji, Yoshinao Oda, Yoshihiko Maehara

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Background: Programmed cell death ligand 1 (PD-L1) expression in lung cancer appears to be important in immunotherapy, as its expression can predict responses to programmed cell death-1 (PD-1)-blocking antibodies. However, a definitive antibody and cut-off value for PD-L1 expression are urgently needed. Materials and Methods: The PD-L1 expression in 40 surgically resected small cell lung cancer (SCLC) specimens was evaluated by immunohistochemistry (IHC) with three different antibodies: clones E1L3N, 28-8, and SP142, and using three different evaluations: the Allred score, 1% cut-off, and 5% cut-off. Results: The percentage of tumors with positive PD-L1 expression was inconsistent in the IHC evaluations using the Allred score and 1% cut-off. However, the IHC evaluations using the 5% cut-off showed similar rates of expression using the three different antibodies. Conclusion: The results of this study provided detailed evidence on the frequency of PD-L1 expression in surgically resected SCLC, which may be a useful reference for identifying patients with PD-L1-expressing SCLC.

Original languageEnglish
Pages (from-to)3409-3412
Number of pages4
JournalAnticancer research
Volume36
Issue number7
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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