An improved mounting method for observation of thick specimens using confocal microscopy

M. Kagayama, Y. Sasano, Masato Hirata, I. Mizoguchi, Ichiro Takahashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.

Original languageEnglish
Pages (from-to)231-233
Number of pages3
JournalBiotechnic and Histochemistry
Volume71
Issue number5
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Confocal Microscopy
Air
Observation
Immersion
Lenses
Glass
Oils
Fluorescence
spurr resin

All Science Journal Classification (ASJC) codes

  • Histology
  • Medical Laboratory Technology

Cite this

An improved mounting method for observation of thick specimens using confocal microscopy. / Kagayama, M.; Sasano, Y.; Hirata, Masato; Mizoguchi, I.; Takahashi, Ichiro.

In: Biotechnic and Histochemistry, Vol. 71, No. 5, 01.01.1996, p. 231-233.

Research output: Contribution to journalArticle

Kagayama, M. ; Sasano, Y. ; Hirata, Masato ; Mizoguchi, I. ; Takahashi, Ichiro. / An improved mounting method for observation of thick specimens using confocal microscopy. In: Biotechnic and Histochemistry. 1996 ; Vol. 71, No. 5. pp. 231-233.
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