An in vitro system for screening anti-hepatitis b virus drugs

Keiji Ueda, Toshiki Tsurimoto, Takemitsu Nagahata, Osamu Chisaka, Kenichi Matsubarai

Research output: Contribution to journalArticle

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Abstract

A human hepatoblastoma cell line (HB 611) that continuously synthesizes hepatitis B viral (HBV) DNA was grown in the presence of various inhibitors of DNA synthesis, and the DNA from the cells was analyzed by the Southern blotting method to examine selective inhibition of the viral DNA synthesis. Among those that showed selective inhibition, and interferons α and β, acyclovir, and dideoxy cytidine were effective. This system should be useful for screening new antiviral agents against HBV.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalVirology
Volume169
Issue number1
DOIs
Publication statusPublished - Jan 1 1989

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Hepatitis Viruses
Viral DNA
Hepatitis B
Nucleic Acid Synthesis Inhibitors
Hepatoblastoma
Cytidine
Acyclovir
Southern Blotting
Pharmaceutical Preparations
Interferons
Antiviral Agents
Cell Line
DNA
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

An in vitro system for screening anti-hepatitis b virus drugs. / Ueda, Keiji; Tsurimoto, Toshiki; Nagahata, Takemitsu; Chisaka, Osamu; Matsubarai, Kenichi.

In: Virology, Vol. 169, No. 1, 01.01.1989, p. 213-216.

Research output: Contribution to journalArticle

Ueda, K, Tsurimoto, T, Nagahata, T, Chisaka, O & Matsubarai, K 1989, 'An in vitro system for screening anti-hepatitis b virus drugs', Virology, vol. 169, no. 1, pp. 213-216. https://doi.org/10.1016/0042-6822(89)90057-3
Ueda, Keiji ; Tsurimoto, Toshiki ; Nagahata, Takemitsu ; Chisaka, Osamu ; Matsubarai, Kenichi. / An in vitro system for screening anti-hepatitis b virus drugs. In: Virology. 1989 ; Vol. 169, No. 1. pp. 213-216.
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