An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer: A culture system based on collagen gel embedding

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages135-141
Number of pages7
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Pancreatic Neoplasms
Collagen
Gels
Fats
Stromal Cells
Adipose Tissue
Neoplasms
Adenocarcinoma
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer : A culture system based on collagen gel embedding. / Okumura, Takashi; Ouchida, Kenoki; Nakamura, Masafumi.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 135-141 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingChapter

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