TY - JOUR
T1 - An inhibitory effect of actin on casein kinase II activity in vitro
AU - Karino, Atsushi
AU - Tanoue, Shin
AU - Fukuda, Motoko
AU - Nakamura, Takeshi
AU - Ohtsuki, Kenzo
N1 - Funding Information:
Acknowledgements." This work was supported in part by grants from Kitasato University (AKRP-1995) and the Ministry of Education, Science and Culture of Japan (Grant-in-Aid No. 08670532, 1996). We are grateful to Dr. S. Bishayee (New Jersey Medical School, NJ, USA) for analytical experiments on an actin-binding assay, and to Dr. Ian Gleadall (Tohoku University, Sendai 980-77, Japan) for critical comments on the manuscript.
PY - 1996/12/2
Y1 - 1996/12/2
N2 - The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of α-monomeric CK-II (CK-IIα) as well as oligomeric CK-II (α2β2) in a dose-dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin-induced inhibition of CK-II activity is due to the binding of actin to the α-subunit of CK-II and is non-competitive with its phosphate acceptors. In addition, it is demonstrated that actin binds directly to CK-II: both actin and CK-II are coprecipitated by anti-serum against Drosophila CK-IIβ or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK-II-mediated signal transduction.
AB - The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of α-monomeric CK-II (CK-IIα) as well as oligomeric CK-II (α2β2) in a dose-dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin-induced inhibition of CK-II activity is due to the binding of actin to the α-subunit of CK-II and is non-competitive with its phosphate acceptors. In addition, it is demonstrated that actin binds directly to CK-II: both actin and CK-II are coprecipitated by anti-serum against Drosophila CK-IIβ or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK-II-mediated signal transduction.
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U2 - 10.1016/S0014-5793(96)01266-5
DO - 10.1016/S0014-5793(96)01266-5
M3 - Article
C2 - 8977130
AN - SCOPUS:0030566861
SN - 0014-5793
VL - 398
SP - 317
EP - 321
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -