An inhibitory effect of actin on casein kinase II activity in vitro

Atsushi Karino, Shin Tanoue, Motoko Fukuda, Takeshi Nakamura, Kenzo Ohtsuki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of α-monomeric CK-II (CK-IIα) as well as oligomeric CK-II (α2β2) in a dose-dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin-induced inhibition of CK-II activity is due to the binding of actin to the α-subunit of CK-II and is non-competitive with its phosphate acceptors. In addition, it is demonstrated that actin binds directly to CK-II: both actin and CK-II are coprecipitated by anti-serum against Drosophila CK-IIβ or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK-II-mediated signal transduction.

Original languageEnglish
Pages (from-to)317-321
Number of pages5
JournalFEBS Letters
Volume398
Issue number2-3
DOIs
Publication statusPublished - Dec 2 1996

Fingerprint

Casein Kinase II
Actins
Ascaris suum
Signal transduction
Phosphorylation
In Vitro Techniques
Mitogen-Activated Protein Kinase Kinases
Protein Kinases
Drosophila
Muscle
Signal Transduction
Phosphotransferases
Immunoglobulin G
Phosphates
Muscles

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

An inhibitory effect of actin on casein kinase II activity in vitro. / Karino, Atsushi; Tanoue, Shin; Fukuda, Motoko; Nakamura, Takeshi; Ohtsuki, Kenzo.

In: FEBS Letters, Vol. 398, No. 2-3, 02.12.1996, p. 317-321.

Research output: Contribution to journalArticle

Karino, Atsushi ; Tanoue, Shin ; Fukuda, Motoko ; Nakamura, Takeshi ; Ohtsuki, Kenzo. / An inhibitory effect of actin on casein kinase II activity in vitro. In: FEBS Letters. 1996 ; Vol. 398, No. 2-3. pp. 317-321.
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