An intermediate of ubiquinone biosynthesis exists in the microsomal fraction of hepG2 cells

Dongchon Rang, Koichiro Takeshige, Shigeki Minakami

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion framgents of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universally radiolabeled [14C] -p-hydroxybenzoate, a precursor of ubiquinone, was incorporated into the compound on incubation with the cells, suggesting that the compound is a precursor of ubiquinone. The presence of the compound in the microsomal fraction of HepG2 cells was not due to contamination by the mitochondrial fraction because the activity of succinate-cytochrome c reductase in the microsomal fraction was below 1% of that in the mitochondrial fraction, whereas the contents of ubiquinone and the compound in the former were 4.6 and 7.8% of those in the latter, respectively. These results support the hypothesis that ubiquinone biosynthesis might occur in microsomes as well as mitochondria.

Original languageEnglish
Pages (from-to)893-895
Number of pages3
JournalJournal of biochemistry
Volume108
Issue number6
DOIs
Publication statusPublished - Dec 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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