An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis

Makoto Takahashi, Kazuhiko Nakamura, Kuniomi Honda, Yousuke Kitamura, Takahiro Mizutani, Yuzuru Araki, Teppei Kabemura, Yoshiharu Chijiiwa, Naohiko Harada, Hajime Nawata

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Evidence suggests that CD4+CD25+ regulatory T cells play a crucial role in the suppression of intestinal inflammation. However, their role in the suppression of inflammatory bowel disease has not yet been addressed. We examined the proportion of regulatory T cells in inflammatory bowel disease. First, we isolated CD4+CD45RO+CD25+ T cells from the peripheral blood of healthy persons and showed that these cells suppressed T cell proliferation profoundly and expressed FoxP3 abundantly, revealing that they are regulatory cells. Then the proportion of CD45RO+CD25+ in peripheral blood CD4+ T cells was analyzed in patients and healthy controls by flow cytometry. CD4+CD45RO+CD25+ T cell frequency was significantly lower in active ulcerative colitis than in the control and inactive ulcerative colitis. CD4+CD45RO+CD25+ T cell frequency was inversely correlated with the clinical and endoscopic severity of ulcerative colitis. These results suggest that a deficiency of regulatory T cells is associated with the progression of ulcerative colitis.

Original languageEnglish
Pages (from-to)677-686
Number of pages10
JournalDigestive Diseases and Sciences
Volume51
Issue number4
DOIs
Publication statusPublished - Apr 1 2006

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Regulatory T-Lymphocytes
Ulcerative Colitis
T-Lymphocytes
Inflammatory Bowel Diseases
Flow Cytometry
Cell Proliferation
Inflammation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis. / Takahashi, Makoto; Nakamura, Kazuhiko; Honda, Kuniomi; Kitamura, Yousuke; Mizutani, Takahiro; Araki, Yuzuru; Kabemura, Teppei; Chijiiwa, Yoshiharu; Harada, Naohiko; Nawata, Hajime.

In: Digestive Diseases and Sciences, Vol. 51, No. 4, 01.04.2006, p. 677-686.

Research output: Contribution to journalArticle

Takahashi, M, Nakamura, K, Honda, K, Kitamura, Y, Mizutani, T, Araki, Y, Kabemura, T, Chijiiwa, Y, Harada, N & Nawata, H 2006, 'An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis', Digestive Diseases and Sciences, vol. 51, no. 4, pp. 677-686. https://doi.org/10.1007/s10620-006-3191-2
Takahashi, Makoto ; Nakamura, Kazuhiko ; Honda, Kuniomi ; Kitamura, Yousuke ; Mizutani, Takahiro ; Araki, Yuzuru ; Kabemura, Teppei ; Chijiiwa, Yoshiharu ; Harada, Naohiko ; Nawata, Hajime. / An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis. In: Digestive Diseases and Sciences. 2006 ; Vol. 51, No. 4. pp. 677-686.
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