An isotope effect on the comparative quantification of flavonoids by means of methylation-based stable isotope dilution coupled with capillary liquid chromatography/mass spectrometry

EI'Ichiro Fukusaki, Kazuo Harada, Takeshi Bamba, Akio Kobayashi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Ionization suppression is a serious problem in liquid chromatography/mass spectrometry-based metabolomics, and stable isotope dilution-based comparative quantification is one of the most important methods of overcoming this problem. Herein, the use of [13C]-methylation-based stable isotope dilution for comparative quantification of flavonoids is demonstrated. This is in contrast to the equivalent deuterium labeling methylation method, which has an adverse isotope effect on reverse phase chromatography.

Original languageEnglish
Pages (from-to)75-77
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number1
DOIs
Publication statusPublished - Jan 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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