An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines

Osamu Morinaga, Ayako Fujino, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An on-membrane quantitative analysis system has been developed for determining glycyrrhizin (GC) in licorice roots and traditional Chinese medicines. A GC standard and the extracts of licorice roots and traditional Chinese medicines were applied to a polyethersulfone (PES) membrane and were developed by acetonitrile/water/formic acid (45:55:2, by volume), then treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a GC-BSA conjugate on a PES membrane. Anti-GC monoclonal antibody was bound and then a second antibody labeled with peroxidase directed against the first antibody. Finally a substrate reacted with the enzyme and gave staining. The stained membrane was scanned and coloring spots were analyzed quantitatively using graphic analysis by NIH Image software, indicating at least 0.5 μg of GC was clearly detectable. GC can be analyzed quantitatively between 1.0 and 8.0 μg.

Original languageEnglish
Pages (from-to)668-672
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume383
Issue number4
DOIs
Publication statusPublished - Oct 1 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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