An oral introduction of intestinal bacteria prevents the development of a long-term Th2-skewed immunological memory induced by neonatal antibiotic treatment in mice

N. Sudo, X. N. Yu, Y. Aiba, N. Oyama, J. Sonoda, Y. Koga, C. Kubo

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Background: Recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. Our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the Thl/Th2 balance towards a Th2-dominant immunity. Objective: The first purpose of this study is to clarify whether or not the supplementation of intestinal bacteria can reverse such a Th2-skewed response induced by neonatal antibiotic use. The second objective is to elucidate the contribution of genetic factors to antibiotic-induced immunedeviation. Methods: BALB/c or C57BL/6 mice at 3 weeks of age were orally administered 600 μg/day of kanamycin sulphate for seven consecutive days. Thereafter, the mice were inoculated with one type of intestinal bacterial species: Enterococcus faecalis, Lactobacillus acidophilus or Bacteroides vulgatus. Blood samples were collected 10 weeks after the cessation of kanamycin treatment, and the effect of the kanamycin treatment on Thl/Th2 balance was evaluated based on in vivo antibody levels. Results: A kanamycin-induced elevation of the serum IgE levels was reversed by the supplementation with Enterococcus faecalis, and to a lesser extent by that with Lactobacillus acidophilus. The IgE/IgG2a ratio in the mice supplemented with Enterococcus faecalis significantly decreased in comparison with that in the kanamycin-treated mice without any bacterial supplementation, while such a ratio was enhanced in the mice inoculated with Bacteroides vulgatus. No antibiotic-induced Th2-skewed response was seen in C57BL/6 mice that are genetically biased towards Thl-dominant immunity. Conclusion: These results suggest that adequate probiotic intervention after antibiotic treatment may improve the intestinal ecosystem, and thereby prevent the Th2-shifted immunity induced by neonatal antibiotic use. In addition, the difference of genetic backgrounds also contributes to such an antibiotic-induced Th2-skewed response.

Original languageEnglish
Pages (from-to)1112-1116
Number of pages5
JournalClinical and Experimental Allergy
Volume32
Issue number7
DOIs
Publication statusPublished - Jul 27 2002

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Immunologic Memory
Kanamycin
Anti-Bacterial Agents
Bacteria
Enterococcus faecalis
Lactobacillus acidophilus
Immunity
Bacteroides
Inbred C57BL Mouse
Immunoglobulin E
Therapeutics
Withholding Treatment
Probiotics
Ecosystem
Epidemiologic Studies
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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An oral introduction of intestinal bacteria prevents the development of a long-term Th2-skewed immunological memory induced by neonatal antibiotic treatment in mice. / Sudo, N.; Yu, X. N.; Aiba, Y.; Oyama, N.; Sonoda, J.; Koga, Y.; Kubo, C.

In: Clinical and Experimental Allergy, Vol. 32, No. 7, 27.07.2002, p. 1112-1116.

Research output: Contribution to journalArticle

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abstract = "Background: Recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. Our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the Thl/Th2 balance towards a Th2-dominant immunity. Objective: The first purpose of this study is to clarify whether or not the supplementation of intestinal bacteria can reverse such a Th2-skewed response induced by neonatal antibiotic use. The second objective is to elucidate the contribution of genetic factors to antibiotic-induced immunedeviation. Methods: BALB/c or C57BL/6 mice at 3 weeks of age were orally administered 600 μg/day of kanamycin sulphate for seven consecutive days. Thereafter, the mice were inoculated with one type of intestinal bacterial species: Enterococcus faecalis, Lactobacillus acidophilus or Bacteroides vulgatus. Blood samples were collected 10 weeks after the cessation of kanamycin treatment, and the effect of the kanamycin treatment on Thl/Th2 balance was evaluated based on in vivo antibody levels. Results: A kanamycin-induced elevation of the serum IgE levels was reversed by the supplementation with Enterococcus faecalis, and to a lesser extent by that with Lactobacillus acidophilus. The IgE/IgG2a ratio in the mice supplemented with Enterococcus faecalis significantly decreased in comparison with that in the kanamycin-treated mice without any bacterial supplementation, while such a ratio was enhanced in the mice inoculated with Bacteroides vulgatus. No antibiotic-induced Th2-skewed response was seen in C57BL/6 mice that are genetically biased towards Thl-dominant immunity. Conclusion: These results suggest that adequate probiotic intervention after antibiotic treatment may improve the intestinal ecosystem, and thereby prevent the Th2-shifted immunity induced by neonatal antibiotic use. In addition, the difference of genetic backgrounds also contributes to such an antibiotic-induced Th2-skewed response.",
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N2 - Background: Recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. Our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the Thl/Th2 balance towards a Th2-dominant immunity. Objective: The first purpose of this study is to clarify whether or not the supplementation of intestinal bacteria can reverse such a Th2-skewed response induced by neonatal antibiotic use. The second objective is to elucidate the contribution of genetic factors to antibiotic-induced immunedeviation. Methods: BALB/c or C57BL/6 mice at 3 weeks of age were orally administered 600 μg/day of kanamycin sulphate for seven consecutive days. Thereafter, the mice were inoculated with one type of intestinal bacterial species: Enterococcus faecalis, Lactobacillus acidophilus or Bacteroides vulgatus. Blood samples were collected 10 weeks after the cessation of kanamycin treatment, and the effect of the kanamycin treatment on Thl/Th2 balance was evaluated based on in vivo antibody levels. Results: A kanamycin-induced elevation of the serum IgE levels was reversed by the supplementation with Enterococcus faecalis, and to a lesser extent by that with Lactobacillus acidophilus. The IgE/IgG2a ratio in the mice supplemented with Enterococcus faecalis significantly decreased in comparison with that in the kanamycin-treated mice without any bacterial supplementation, while such a ratio was enhanced in the mice inoculated with Bacteroides vulgatus. No antibiotic-induced Th2-skewed response was seen in C57BL/6 mice that are genetically biased towards Thl-dominant immunity. Conclusion: These results suggest that adequate probiotic intervention after antibiotic treatment may improve the intestinal ecosystem, and thereby prevent the Th2-shifted immunity induced by neonatal antibiotic use. In addition, the difference of genetic backgrounds also contributes to such an antibiotic-induced Th2-skewed response.

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