An oscillation engineering of enzymes - Ultrasound regulation of enzyme activities

Takayoshi Kawasaki, Yu Hoshino, Hidemi Isobe, Yoshio Okahata

Research output: Contribution to conferencePaper

Abstract

Although it has been suggested that the flexibility and conformational mobility of protein may be essential for catalysis, little mechanical evidence has been demonstrated. In this study, we applied frequency specified ultrasound waves into the enzyme reaction systems in order to control enzyme functions by modulating the polypeptide dynamics. In this study, we investigated the ultrasound effects on reactivities of thermolysin and DNA polymerase. In the case of thermolysin, irradiation of ultrasound with frequency range of several hundred kHz leads to reduce the Km value, and therefore enhances the overall reactivity of the thermolysin. In order to examine the ultrasound effect on DNA polymerase, we utilized the quartz crystal microbalance (QCM) system. The binding kinetics of Klenow Fragment (KF) onto a template/primer DNA complex immobilized on the QCM was monitored as mass changes. The reaction kinetics of KF bound to the template/primer DNA was also studied with the same manner. Frequency dependencies on each reaction step were found.

Original languageEnglish
Pages5073-5074
Number of pages2
Publication statusPublished - Dec 1 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period9/20/069/22/06

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Kawasaki, T., Hoshino, Y., Isobe, H., & Okahata, Y. (2006). An oscillation engineering of enzymes - Ultrasound regulation of enzyme activities. 5073-5074. Paper presented at 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan.