An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

Madan Kumar Paudel, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Wogonin glucuronide (wogonin 7-O-β-d-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.

Original languageEnglish
Pages (from-to)703-710
Number of pages8
JournalJournal of Natural Medicines
Volume71
Issue number4
DOIs
Publication statusPublished - Oct 1 2017

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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