An SPR sensor chip based on peptide-modified single-walled carbon nanotubes with enhanced sensitivity and selectivity in the detection of 2,4,6-trinitrotoluene explosives

Jin Wang, Sanyang Du, Takeshi Onodera, Rui Yatabe, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

Research output: Contribution to journalArticle

6 Citations (Scopus)


In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide–SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide–SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips.

Original languageEnglish
Article number4461
JournalSensors (Switzerland)
Issue number12
Publication statusPublished - Dec 1 2018


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

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