An SPR sensor chip based on peptide-modified single-walled carbon nanotubes with enhanced sensitivity and selectivity in the detection of 2,4,6-trinitrotoluene explosives

Jin Wang, Sanyang Du, Takeshi Onodera, Rui Yatabe, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

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6 Citations (Scopus)

Abstract

In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide–SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide–SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips.

Original languageEnglish
Article number4461
JournalSensors (Switzerland)
Volume18
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

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