An ultrastructural study of the bone-titanium interface using pure titanium-coated plastic and pure titanium rod implants

Yasunori Ayukawa, Fumitaka Takeshita, Takashi Inoue, Masao Yoshinari, Yoshiro Ohtsuka, Kenji Murai, Masaki Shimono, Tsuneo Suetsugu, Teruo Tanaka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The present study was designed to examine the bone-titanium interface of the titanium-coated or titanium rod implants inserted for 28 days in the tibiae of 6-week-old rats. Basically, there were hardly differences in the interfaces between the titanium-coated and titanium rod implants. Light microscopically, titanium layer appeared to make direct contact with the mature bone or poorly mineralized layer, and one or a few layers of slender cells were localized contacting the titanium. Ultrastructurally, titanium came in direct contact with either the bone, the poorly mineralized layer consisting of delicate fibril-like structures or the slender cell layer through a thin amorphous zone (2040 nm). This amorphous zone was positive for ruthenium red which has a high affinity for proteoglycans. Part of the interfacial slender cells had abundant rough-ER and glycogen granules as osteoblasts, while some slender cells had well-developed rough-ER but no glycogen, and often endocytosed the titanium fragments, as shown in the fibroblasts. These findings suggest that an amorphous zone consisting of proteoglycans is thus essential for attachment between the slender cell layer, poorly mineralized layer or mature bone and titanium. Furthermore, slender cells close to the implant may also be considered to function as either osteoblasts for bone production adjacent to the implant or as scavengers for the removal of interfacial debris at the bone-titanium interface.

Original languageEnglish
Pages (from-to)243-254
Number of pages12
JournalActa Histochemica et Cytochemica
Volume29
Issue number3
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Titanium
Plastics
Bone
Bone and Bones
Osteoblasts
Proteoglycans
Glycogen
Cells
Ruthenium Red
Fibroblasts
Endocytosis
Tibia
Debris
Rats
Light

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

An ultrastructural study of the bone-titanium interface using pure titanium-coated plastic and pure titanium rod implants. / Ayukawa, Yasunori; Takeshita, Fumitaka; Inoue, Takashi; Yoshinari, Masao; Ohtsuka, Yoshiro; Murai, Kenji; Shimono, Masaki; Suetsugu, Tsuneo; Tanaka, Teruo.

In: Acta Histochemica et Cytochemica, Vol. 29, No. 3, 01.01.1996, p. 243-254.

Research output: Contribution to journalArticle

Ayukawa, Y, Takeshita, F, Inoue, T, Yoshinari, M, Ohtsuka, Y, Murai, K, Shimono, M, Suetsugu, T & Tanaka, T 1996, 'An ultrastructural study of the bone-titanium interface using pure titanium-coated plastic and pure titanium rod implants', Acta Histochemica et Cytochemica, vol. 29, no. 3, pp. 243-254. https://doi.org/10.1267/ahc.29.243
Ayukawa, Yasunori ; Takeshita, Fumitaka ; Inoue, Takashi ; Yoshinari, Masao ; Ohtsuka, Yoshiro ; Murai, Kenji ; Shimono, Masaki ; Suetsugu, Tsuneo ; Tanaka, Teruo. / An ultrastructural study of the bone-titanium interface using pure titanium-coated plastic and pure titanium rod implants. In: Acta Histochemica et Cytochemica. 1996 ; Vol. 29, No. 3. pp. 243-254.
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