An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats

Yuki Kimura, Tetsu Sakai, Makoto Takeuchi, Yoshihiro Matsumoto, Kouichi Watanabe, Masakatsu Yuuki, Toshihiko Takada, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    We have previously shown an age-associated increase in unique CD4+CD8+ intestinal intraepithelial lymphocytes (i-IEL) in rats. To elucidate the potential causes of the increase in CD4+CD8+ i-IEL with age, we analyzed the specificity of the CD4+CD8+ i-IEL and influence of intestinal microflora on the increase in this subset in aged rats. The purified CD4+CD8+ i-IEL proliferated in response to DnaK [Escherichia coli (E. coli) HSP70] in the presence of mitomycin-c (MMC)-treated syngeneic spleen cells. The proportion of CD4+CD8+ T cells in whole i-IEL were significantly increased in aged rats fed commercial (CL-2) diet but not in those fed home-made (purified) diet under conventional condition. No CD4+CD8+ i-IEL were detected in aged rats under germfree condition, irrespective of diet feeding. A larger number of Enterobacteriaceae, especially E. coli, were detected in the intestinal contents and feces from aged rats with CD4+CD8+ i-IEL compared with those from aged rats fed without CD4+CD8+ i-IEL. The unique CD4+CD8+ i-IEL population specific for E. coli HSP may be associated with long term exposure to intestinal E. coli in aged rats.

    Original languageEnglish
    Pages (from-to)550-566
    Number of pages17
    JournalImmunobiology
    Volume196
    Issue number5
    Publication statusPublished - Mar 1 1997

    Fingerprint

    Lymphocytes
    Escherichia coli
    Diet
    Gastrointestinal Microbiome
    Gastrointestinal Contents
    Mitomycin
    Enterobacteriaceae
    Feces
    Spleen
    T-Lymphocytes
    Population

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Hematology

    Cite this

    Kimura, Y., Sakai, T., Takeuchi, M., Matsumoto, Y., Watanabe, K., Yuuki, M., ... Yoshikai, Y. (1997). An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats. Immunobiology, 196(5), 550-566.

    An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats. / Kimura, Yuki; Sakai, Tetsu; Takeuchi, Makoto; Matsumoto, Yoshihiro; Watanabe, Kouichi; Yuuki, Masakatsu; Takada, Toshihiko; Yoshikai, Yasunobu.

    In: Immunobiology, Vol. 196, No. 5, 01.03.1997, p. 550-566.

    Research output: Contribution to journalArticle

    Kimura, Y, Sakai, T, Takeuchi, M, Matsumoto, Y, Watanabe, K, Yuuki, M, Takada, T & Yoshikai, Y 1997, 'An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats', Immunobiology, vol. 196, no. 5, pp. 550-566.
    Kimura, Yuki ; Sakai, Tetsu ; Takeuchi, Makoto ; Matsumoto, Yoshihiro ; Watanabe, Kouichi ; Yuuki, Masakatsu ; Takada, Toshihiko ; Yoshikai, Yasunobu. / An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats. In: Immunobiology. 1997 ; Vol. 196, No. 5. pp. 550-566.
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