Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304

Takanori Matsuda, Keishi Sakaguchi, Rie Hamaguchi, Takumi Kobayashi, Eriko Abe, Yoichiro Hama, Masahiro Hayashi, Daiske Honda, Yuji Okita, Shinichi Sugimoto, Nozomu Okino, Makoto Ito

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Thraustochytrids are known to synthesize PUFAs such as docosahexaenoic acid (DHA). Accumulating evidence suggests the presence of two synthetic pathways of PUFAs in thraustochytrids: the polyketide synthase-like (PUFA synthase) and desaturase/elongase (standard) pathways. It remains unclear whether the latter pathway functions in thraustochytrids. In this study, we report that the standard pathway produces PUFA in Thraustochytrium aureum ATCC 34304. We isolated a gene encoding a putative Δ12-fatty acid desaturase (TauΔ12des) from T. aureum. Yeasts transformed with the tauΔ12des converted endogenous oleic acid (OA) into linoleic acid (LA). The disruption of the tauΔ12des in T. aureum by homologous recombination resulted in the accumulation of OA and a decrease in the levels of LA and its downstream PUFAs. However, the DHA content was increased slightly in tauΔ12des -disruption mutants, suggesting that DHA is primarily produced in T. aureum via the PUFA synthase pathway. The transformation of the tauΔ12des -disruption mutants with a tauΔ12des expression cassette restored the wild-type fatty acid profiles. These data clearly indicate that TauΔ12des functions as Δ12-fatty acid desaturase in the standard pathway of T. aureum and demonstrate that this thraustochytrid produces PUFAs via both the PUFA synthase and the standard pathways.

Original languageEnglish
Pages (from-to)1210-1222
Number of pages13
JournalJournal of Lipid Research
Volume53
Issue number6
DOIs
Publication statusPublished - Jun 1 2012

Fingerprint

Fatty Acid Desaturases
Docosahexaenoic Acids
Linoleic Acid
Oleic Acid
Polyketide Synthases
Gene encoding
Homologous Recombination
Yeast
Fatty Acids
Yeasts
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304. / Matsuda, Takanori; Sakaguchi, Keishi; Hamaguchi, Rie; Kobayashi, Takumi; Abe, Eriko; Hama, Yoichiro; Hayashi, Masahiro; Honda, Daiske; Okita, Yuji; Sugimoto, Shinichi; Okino, Nozomu; Ito, Makoto.

In: Journal of Lipid Research, Vol. 53, No. 6, 01.06.2012, p. 1210-1222.

Research output: Contribution to journalArticle

Matsuda, T, Sakaguchi, K, Hamaguchi, R, Kobayashi, T, Abe, E, Hama, Y, Hayashi, M, Honda, D, Okita, Y, Sugimoto, S, Okino, N & Ito, M 2012, 'Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304', Journal of Lipid Research, vol. 53, no. 6, pp. 1210-1222. https://doi.org/10.1194/jlr.M024935
Matsuda, Takanori ; Sakaguchi, Keishi ; Hamaguchi, Rie ; Kobayashi, Takumi ; Abe, Eriko ; Hama, Yoichiro ; Hayashi, Masahiro ; Honda, Daiske ; Okita, Yuji ; Sugimoto, Shinichi ; Okino, Nozomu ; Ito, Makoto. / Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304. In: Journal of Lipid Research. 2012 ; Vol. 53, No. 6. pp. 1210-1222.
@article{697eb7ae96e1486193b88afc6ab2cdc0,
title = "Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304",
abstract = "Thraustochytrids are known to synthesize PUFAs such as docosahexaenoic acid (DHA). Accumulating evidence suggests the presence of two synthetic pathways of PUFAs in thraustochytrids: the polyketide synthase-like (PUFA synthase) and desaturase/elongase (standard) pathways. It remains unclear whether the latter pathway functions in thraustochytrids. In this study, we report that the standard pathway produces PUFA in Thraustochytrium aureum ATCC 34304. We isolated a gene encoding a putative Δ12-fatty acid desaturase (TauΔ12des) from T. aureum. Yeasts transformed with the tauΔ12des converted endogenous oleic acid (OA) into linoleic acid (LA). The disruption of the tauΔ12des in T. aureum by homologous recombination resulted in the accumulation of OA and a decrease in the levels of LA and its downstream PUFAs. However, the DHA content was increased slightly in tauΔ12des -disruption mutants, suggesting that DHA is primarily produced in T. aureum via the PUFA synthase pathway. The transformation of the tauΔ12des -disruption mutants with a tauΔ12des expression cassette restored the wild-type fatty acid profiles. These data clearly indicate that TauΔ12des functions as Δ12-fatty acid desaturase in the standard pathway of T. aureum and demonstrate that this thraustochytrid produces PUFAs via both the PUFA synthase and the standard pathways.",
author = "Takanori Matsuda and Keishi Sakaguchi and Rie Hamaguchi and Takumi Kobayashi and Eriko Abe and Yoichiro Hama and Masahiro Hayashi and Daiske Honda and Yuji Okita and Shinichi Sugimoto and Nozomu Okino and Makoto Ito",
year = "2012",
month = "6",
day = "1",
doi = "10.1194/jlr.M024935",
language = "English",
volume = "53",
pages = "1210--1222",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "6",

}

TY - JOUR

T1 - Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304

AU - Matsuda, Takanori

AU - Sakaguchi, Keishi

AU - Hamaguchi, Rie

AU - Kobayashi, Takumi

AU - Abe, Eriko

AU - Hama, Yoichiro

AU - Hayashi, Masahiro

AU - Honda, Daiske

AU - Okita, Yuji

AU - Sugimoto, Shinichi

AU - Okino, Nozomu

AU - Ito, Makoto

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Thraustochytrids are known to synthesize PUFAs such as docosahexaenoic acid (DHA). Accumulating evidence suggests the presence of two synthetic pathways of PUFAs in thraustochytrids: the polyketide synthase-like (PUFA synthase) and desaturase/elongase (standard) pathways. It remains unclear whether the latter pathway functions in thraustochytrids. In this study, we report that the standard pathway produces PUFA in Thraustochytrium aureum ATCC 34304. We isolated a gene encoding a putative Δ12-fatty acid desaturase (TauΔ12des) from T. aureum. Yeasts transformed with the tauΔ12des converted endogenous oleic acid (OA) into linoleic acid (LA). The disruption of the tauΔ12des in T. aureum by homologous recombination resulted in the accumulation of OA and a decrease in the levels of LA and its downstream PUFAs. However, the DHA content was increased slightly in tauΔ12des -disruption mutants, suggesting that DHA is primarily produced in T. aureum via the PUFA synthase pathway. The transformation of the tauΔ12des -disruption mutants with a tauΔ12des expression cassette restored the wild-type fatty acid profiles. These data clearly indicate that TauΔ12des functions as Δ12-fatty acid desaturase in the standard pathway of T. aureum and demonstrate that this thraustochytrid produces PUFAs via both the PUFA synthase and the standard pathways.

AB - Thraustochytrids are known to synthesize PUFAs such as docosahexaenoic acid (DHA). Accumulating evidence suggests the presence of two synthetic pathways of PUFAs in thraustochytrids: the polyketide synthase-like (PUFA synthase) and desaturase/elongase (standard) pathways. It remains unclear whether the latter pathway functions in thraustochytrids. In this study, we report that the standard pathway produces PUFA in Thraustochytrium aureum ATCC 34304. We isolated a gene encoding a putative Δ12-fatty acid desaturase (TauΔ12des) from T. aureum. Yeasts transformed with the tauΔ12des converted endogenous oleic acid (OA) into linoleic acid (LA). The disruption of the tauΔ12des in T. aureum by homologous recombination resulted in the accumulation of OA and a decrease in the levels of LA and its downstream PUFAs. However, the DHA content was increased slightly in tauΔ12des -disruption mutants, suggesting that DHA is primarily produced in T. aureum via the PUFA synthase pathway. The transformation of the tauΔ12des -disruption mutants with a tauΔ12des expression cassette restored the wild-type fatty acid profiles. These data clearly indicate that TauΔ12des functions as Δ12-fatty acid desaturase in the standard pathway of T. aureum and demonstrate that this thraustochytrid produces PUFAs via both the PUFA synthase and the standard pathways.

UR - http://www.scopus.com/inward/record.url?scp=84861447355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861447355&partnerID=8YFLogxK

U2 - 10.1194/jlr.M024935

DO - 10.1194/jlr.M024935

M3 - Article

C2 - 22368282

AN - SCOPUS:84861447355

VL - 53

SP - 1210

EP - 1222

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 6

ER -