Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium

Kazuhiko Tsukagoshi, Yukihiro Shikata, Riichiro Nakajima, Masaharu Murata, Mizuo Maeda

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10 -4 M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the SIN ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected.

    Original languageEnglish
    Pages (from-to)1195-1198
    Number of pages4
    Journalanalytical sciences
    Volume18
    Issue number11
    DOIs
    Publication statusPublished - Nov 1 2002

    Fingerprint

    Capillary electrophoresis
    Biopolymers
    Chemiluminescence
    Polymer solutions
    Fluorescein
    Detectors
    DNA
    Fluorescence
    Pancreatic Ribonuclease
    Polymerase chain reaction
    Cytochromes
    Muramidase
    Labeling
    Buffers
    Proteins
    Adenosine Triphosphate

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

    Cite this

    Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium. / Tsukagoshi, Kazuhiko; Shikata, Yukihiro; Nakajima, Riichiro; Murata, Masaharu; Maeda, Mizuo.

    In: analytical sciences, Vol. 18, No. 11, 01.11.2002, p. 1195-1198.

    Research output: Contribution to journalArticle

    Tsukagoshi, Kazuhiko ; Shikata, Yukihiro ; Nakajima, Riichiro ; Murata, Masaharu ; Maeda, Mizuo. / Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium. In: analytical sciences. 2002 ; Vol. 18, No. 11. pp. 1195-1198.
    @article{d492f87f2bee4d5ca5c6d4ec88236ba3,
    title = "Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium",
    abstract = "We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10 -4 M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the SIN ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected.",
    author = "Kazuhiko Tsukagoshi and Yukihiro Shikata and Riichiro Nakajima and Masaharu Murata and Mizuo Maeda",
    year = "2002",
    month = "11",
    day = "1",
    doi = "10.2116/analsci.18.1195",
    language = "English",
    volume = "18",
    pages = "1195--1198",
    journal = "Analytical Sciences",
    issn = "0910-6340",
    publisher = "The Japan Society for Analytical Chemistry",
    number = "11",

    }

    TY - JOUR

    T1 - Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium

    AU - Tsukagoshi, Kazuhiko

    AU - Shikata, Yukihiro

    AU - Nakajima, Riichiro

    AU - Murata, Masaharu

    AU - Maeda, Mizuo

    PY - 2002/11/1

    Y1 - 2002/11/1

    N2 - We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10 -4 M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the SIN ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected.

    AB - We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10 -4 M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the SIN ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected.

    UR - http://www.scopus.com/inward/record.url?scp=0036860227&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0036860227&partnerID=8YFLogxK

    U2 - 10.2116/analsci.18.1195

    DO - 10.2116/analsci.18.1195

    M3 - Article

    C2 - 12458702

    AN - SCOPUS:0036860227

    VL - 18

    SP - 1195

    EP - 1198

    JO - Analytical Sciences

    JF - Analytical Sciences

    SN - 0910-6340

    IS - 11

    ER -